Regulated Operon: leuS

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
leuS
- 3102629..3105043
COG0495J leuS-BAC leuS-STA leuS-STR  

Operon evidence: Genome analysis
Reference: Vander Horn PB & Zahler SA (1992), Grundy FJ & Henkin TM (1993), Lapidus A, et al. (1997), Genbank AF008220
Comments: Based on homology, transcriptional readthrough is expected to occur at the stem-loop upstream of leuS if uncharged leucine tRNA binds to the T-box motif in the nascent mRNA transcript. The tRNA anticodon binds to the CUC codon in the sequence GAACUCACC in the leuS leader mRNA.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
TrnS-Leu2 Positive ND 3105180..3105197 AACGAGGGTGGTACCGCG Vander Horn PB & Zahler SA (1992): HM

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
GAAAGTCTCTCGTCCCTTTTTGGGATGAGGGAGTTTTTTTTAGTTTGG
     >>>>>>>>>>>     <<<<<<<<<<<
3105141..3105167
-2538..-2512
-21.3
leuS
AAAAATCCCCTTTGCCAAAAGGGGATTTTTTTTCATCAGT
     >>>>>>>>    <<<<<<<<
3102597..3102616
13..32
-14.2
leuS

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai