Regulated Operon: trpS

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
trpS
- 1218113..1219105
COG0180J trpS-BAC trpS-STA x0244-BAC  

Operon evidence: Genome analysis
Reference: Chow KC & Wong JT (1988), Condon C, et al. (1996)
Comments: Transcriptional readthrough occurs at the stem-loop upstream of trpS if uncharged tryptophan tRNA binds to the T-box motif in the nascent mRNA transcript. The tRNA anticodon binds to the UGG codon in the sequence GAAUGGACU in the trpS leader mRNA.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
TrnD-Trp Positive ND 1219181..1219204 TGGAATAATCAGGGTGGTACCACG Condon C, et al. (1996): HM

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
ACGCTAATCAAAAAACCGCTCTTTGCAAAGAGCGGTTTTTTTCAGTTGAC
               >>>>>>>>    <<<<<<<<
1218085..1218104
9..28
-13.3
trpS
TGGTACCACGGTTCATTCGTCCCTTTTTTACAGGGGAAGAATGAGCCTTTTTTATTATGTTT
         >>>>>>>>>>>>>>>>      <<<<<<<<<<<<<<<<
1219144..1219181
-1068..-1031
-23.9
trpS

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai