 |
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | acoABCL |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| acoA | yfjK | + | 878337..879338 | acetoin dehydrogenase E1 component (TPP-dependent alpha subunit) | COG1071 | acoA-BAC acoA-STR |
| acoB | yfjJ | + | 879342..880370 | acetoin dehydrogenase E1 component (TPP-dependent beta subunit) | COG0022 | acoB-BAC |
| acoC | yfjI | + | 880384..881580 | acetoin dehydrogenase E2 component (dihydrolipoamide acetyltransferase) | COG0508 | acoC-BAC acoC-STR |
| acoL | yfjH | + | 881601..882977 | acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase) | COG1249 | acoL-BAC |
| Operon evidence: | Northern blotting (4637 bp transcript); transcriptional fusions |
|---|---|
| Reference: | Ali NO, et al. (2001), Reents H, et al. (2006), BSORF |
| Comments: | Northern blotting results in BSORF show an acoABCLR-sspH transcript, an acoR-sspH transcript, and a monocistronic sspH transcript. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| AcoR | Positive | -123:-60 | 878186..878249 | CGAGACAAATGAATCAGTTTGAGACAAAACGAGACACACGTCTCAAACTGTCTCCAAAGTGAAG |
Ali NO, et al. (2001): RG |
| CcpA | Negative | +459:+481 | 878767..878789 | AAAATGTAAGCGTTTGCTTTTTC |
Miwa Y, et al. (2000): RG |
| SigL | Promoter | -34:+6 | 878275..878314 | AAAAGACTGGCACACTTCTTGCATTTATAATGGTGAACCC |
Ali NO, et al. (2001): PE HM |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| AAAAAGCAGGCGCATGGATATAAGGCGCCTGCTTTTTTATTGTTGAA >>>>>>>> <<<<<<<< |
acoL |
|