Regulated Operon: gntRKPZ

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
gntR
+ 4112441..4113172 transcriptional regulator (GntR family) COG1802 gntR-BAC x0770-BAC x1154-BAC x1448-BAC  
gntK
+ 4113165..4114706 gluconate kinase COG1070 gntK-STA  
gntP
+ 4114735..4116081 gluconate permease COG2610 gntP-BAC  
gntZ
+ 4116104..4117510 6-phosphogluconate dehydrogenase COG0362  

Operon evidence: S1 nuclease mapping; Northern blotting (5.5 kb transcript)
Reference: Fujita Y, et al. (1986), Yoshida K, et al. (2000)
Comments: Main termination site, as determined by S1 nuclease mapping, is at position 4117549 (first T in the T stretch TTTTTTTCTT). Fujita et al. show that two internal promoters exist, for unknown sigma factor, upstream of gntZ. Yoshida shows a monocistronic 2.0 kb gntZ transcript.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CcpA Negative +138:+160 4112544..4112566 TGATTGAAAGCGGTACCATTTTA Miwa Y, et al. (1993): SDM S1 HB
Chauvaux S, et al. (1998): DB RG
Miwa Y, et al. (2000): RG
Kim JH, et al. (2005): FT
CcpB Negative ND ND ND Chauvaux S, et al. (1998): DB RG
GntR Negative -10:+12 4112397..4112418 ATACTTGTATACAAGTATACTC Yoshida K, et al. (1995): SDM GS FT
SigA Promoter -47:+16 4112360..4112422 ATGAAAGTGTTTGCATAAAAGAAATATTCACGTTATCATACTTGTATACAAGTATACTCCTTG Fujita Y, et al. (1986): S1
SigA Promoter -42:+26 4116036..4116103 CGTTGCTGGACTGGGATTCATTTTATTGTTAAGTTTAGTTGTATGAAATTAAGAAGGAGCTGTAACAC Fujita Y, et al. (1986): S1
unknown Promoter -49:+19 4116036..4116103 CGTTGCTGGACTGGGATTCATTTTATTGTTAAGTTTAGTTGTATGAAATTAAGAAGGAGCTGTAACAC Fujita Y, et al. (1986): S1

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAACACGGTCAGTTTCAACTGAACCGTGTTTTTTTCTTCTATC
     >>>>>>>>>>    <<<<<<<<<<<
4117524..4117548
14..38
-14.2
 gntZ

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai