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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | nasBCDEF |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| nasB | nasBA | - | 360002..362314 | assimilatory nitrate reductase (electron transfer subunit) | COG1251 | |
| nasC | narB, nasBB | - | 357863..359995 | assimilatory nitrate reductase (catalytic subunit) | COG0243 | |
| nasD | nasBC, nirB | - | 355324..357741 | assimilatory nitrite reductase (subunit) | COG1251 | nirB-BAC |
| nasE | nasBD, nirD | - | 354972..355292 | assimilatory nitrite reductase (subunit) | COG2146 | |
| nasF | nasBE | - | 353460..354911 | uroporphyrin-III C-methyltransferase | COG1587 |
| Operon evidence: | Northern blotting (9.2 kb transcript) |
|---|---|
| Reference: | Nakano MM, et al. (1995), Nakano MM, et al. (1998), Yoshida K, et al. (2003) |
| Comments: | The internal promoter in front of nasD leads to a 4.5 kb transcript |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| GlnR | Negative | -58:-42 | 362395..362413 | GTGTAAGTTTTTGTGACAC |
Nakano MM, et al. (1995): DP SDM |
| SigA | Promoter | -49:+17 | 362338..362403 | TTGTGACACGTTTAATGCGTTAACAATGCATTGTGACATAATTTTTAATAGGAGAAAACTTACGAG |
Nakano MM, et al. (1995): PE |
| TnrA | Positive | -58:-42 | 362395..362413 | GTGTAAGTTTTTGTGACAC |
Nakano MM, et al. (1995): DP SDM Yoshida K, et al. (2003): AR HM GS |
| ResD | Positive | -83:-39 | 357811..357854 | TAAAATTTTTAGAACTTTTCGTATATTTTGTTACATTTTATAAC |
Nakano MM, et al. (1998): DB Nakano MM, et al. (2001): RG |
| unknown | Promoter | -50:+6 | 357766..357821 | CATTTTATAACATGTATCTTAAATATTCCTTTTTGCTGTGCTACGCTGGAAAAAAC |
Nakano MM, et al. (1998): PE RG |
| TnrA | Positive | -55:-39 | 357810..357826 | TGTTACATTTTATAACA |
Nakano MM, et al. (1998): RG DB Yoshida K, et al. (2003): AR HM GS |
| ArfM | Positive | ND | ND | ND |
Marino M, et al. (2001): RG DB |
| NsrR | Negative | ND | ND | ND |
Nakano MM, et al. (2006): RG |
| Fur | Negative | ND | 355293..355342 | TGTCGTGACCACATCATAATGACAAAAACTATCATTGAGGAGGAATGGAC |
Baichoo N, et al. (2002): FT |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| GCAAGCAGCCCTTTTCCTCAAGGGCTGTTTTATTTATGCACC >>>>>>>> <<<<<<<< |
nasF |
|