Regulated Operon: nrdIEF-ymaB

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
ymaA nrdI + 1867812..1868204
COG1780 ymaA-BAC nrdI-STA ymaA-BAC  
nrdE
+ 1868164..1870266 ribonucleoside-diphosphate reductase (major subunit) COG0209 nrdE-MYC nrdE-STR  
nrdF
+ 1870284..1871273 ribonucleoside-diphosphate reductase (minor subunit) COG0208 nrdF-MYC nrdF-STA nrdF-STR  
ymaB
+ 1871323..1871943
COG4112  

Operon evidence: Northern blotting (4.1 kb transcript)
Reference: Scotti C, et al. (1996), Hartig E, et al. (2006), Genbank Z68500
Comments: The readthrough terminator downstream of nrdF leads to a 3.4 kb nrdIEF transcript.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
ResD Positive -69:-44 1867695..1867720 AAAATATGAATTTTTCATAAAAACGA Hartig E, et al. (2006): RG DB SDM
SigA Promoter -46:+9 1867718..1867772 CGAATCTATATATTGTGTTTTTGCTAGAGGGTAGTATACTATATATAGTGTTAGT Hartig E, et al. (2006): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
TGAAGATGAAAAAGAGCAGATATAATATCTGCTCTTTTCGGCTTGAAGG
             >>>>>>>>>   <<<<<<<<<
1871262..1871282
-11..9
-14.1
nrdF
TTCTTGAATAAAAAAACCCGGTTTCTCGCGATGAGGAGCCGGGTTTTTTTATGAGACG
                >>>>>>>>>>>     <<<<<<<<<<<
1871949..1871975
6..32
-21.0
ymaB

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai