Regulated Operon: pabBAC-sul-folBK-yazB-yacF-lysS

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
pabB pab + 82861..84273 para-aminobenzoate synthase (subunit A) COG0147 pabB-BAC  
pabA trpG, trpX + 84287..84871 anthranilate synthase (subunit II) COG0512 pabA-BAC x0931-STA  
pabC
+ 84871..85752 aminodeoxychorismate lyase COG0115  
sul
+ 85734..86591 dihydropteroate synthase COG0294  
folB folA, yacE + 86584..86946 dihydroneopterin aldolase COG1539  
folK
+ 86943..87446 7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase COG0801  
yazB
+ 87398..87607
COG1396  
yacF
+ 87631..88632
COG0042 x1602-BAC  
lysS
+ 88724..90223 lysyl-tRNA synthetase COG1190 lysS-BAC-1 lysS-BAC-2  

Operon evidence: Northern blotting (7.5 kb transcript)
Reference: De Sazieu A, et al. (1997), BSORF
Comments: Readthrough terminators after pabA (undefined) and yacF result in a 2.1 kb transcript and a 5.9 kb transcript, respectively. Promoter deletions, reporter gene fusions, and Northern blotting (1.5 kb transcript) suggested the existence of an internal promoter in front of lysS. The MtrB product (TRAP) causes translational attenuation at the pabA ribosomal binding site under conditions of excess tryptophan.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -43:+7 82785..82834 GAAATTCACTTTTTTCACTAACAACATTGCTTTACAATTAAAAACAAGTA De Saizieu A, et al. (1997): PE RG NB
MtrB Negative ND 84233..84284 GAGCATTAGAGCTGAGCGAAGAAGAGACAAAAATTAGATGAGGTGAGCGGAG Kane JF (1977): DB, anthranilate synthase activity, tryptophan excretion measurement
Babitzke P, et al. (1994): filter binding assay
Yang M, et al. (1995): RG DP SDM
Lee AI, et al. (1996): DB RG
Du H, et al. (1997): FT, in-vitro translation
Valbuzzi A & Yanofsky C (2001): DB RG
Yakhnin H, et al. (2004): RG DB GS

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AAAAAGAGCGGTATCCTCCATAGGGAAAGGATGCCGCTCTTTTTAAATCCCTTA
     >>>>>>>>>>>>>>    <<<<<<<<<<<<<<<<
90231..90264
8..41
-25.3
lysS
GCTCACTGCTAGTTTTACGCTGGCAGTTTTTCTGCTTTTTT
     >>>>>>>>     <<<<<<<<
88656..88676
24..44
-13.0
yacF

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai