Regulated Operon: phoB-ydhF

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
phoB phoAIII - 619643..621031 alkaline phosphatase III COG1785 phoB-STA x1451-BAC  
ydhF
- 618867..619577

 

Operon evidence: Northern blotting (2.2 kb transcript)
Reference: Antelmann H, et al. (2000)
Comments:


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
PhoP Positive (SigA promoter); Negative (SigE promoter) -65:-7 (SigA); +18:+76 (SigE) 621075..621133 TCTTGTAAATCCAATCTTTAAAATCGATTAATACTAGCTTAACAGTTTAAAAATATAAT Liu W, et al. (1997): GS FT
Abdel-Fattah WR, et al. (2005): RG; in-vitro transcription assay; FT
Allenby NE, et al. (2005): DB AR
PhoP Negative (SigE promoter) -35:+14 621137..621185 CTAAAACTTCTCATAAAAGAATAACCATTATTTAAGGGTGCCAGTTCAT Abdel-Fattah WR, et al. (2005): RG; in-vitro transcription assay; FT
SigA Promoter -45:+4 621065..621113 AAATCGATTAATACTAGCTTAACAGTTTAAAAATATAATTGGGTTGTCA Chesnut RS, et al. (1991): PE DP
Abdel-Fattah WR, et al. (2005): RG
Allenby NE, et al. (2005): DB AR
SigE Promoter -35:+14 621137..621185 CTAAAACTTCTCATAAAAGAATAACCATTATTTAAGGGTGCCAGTTCAT Chesnut RS, et al. (1991): PE DP
Abdel-Fattah WR, et al. (2005): RG; in-vitro transcription assay

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
ATCAAGAACTCCCGTACAAGGTACGGGAGTTCTTTTTCTTATTTGTT
     >>>>>>>>>>>>   <<<<<<<<<<<<
618835..618861
6..32
-22.2
ydhF

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai