Regulated Operon: pssA-ybfM-psd

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
pssA pss + 247732..248265 phosphatidylserine synthase COG1183 x1012-BAC  
ybfM
+ 248256..248744
COG0586  
psd
+ 248737..249528 phosphatidylserine decarboxylase; 32 kDa precursor processed into a 29 kDa protein COG0688  

Operon evidence: Northern blotting (1.9 kb transcript)
Reference: Cao M & Helmann JD (2004), Matsumoto K, et al. (1998)
Comments: Matsumoto et al. found a terminator-like sequence inside ybfM; its significance is unknown.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter ND 247502..247547 GCGTCTTGTCCTTTTTATGCGGGGGGATAAAATAGTAAGGAACATG Cao M & Helmann JD (2004): ROMA RG
SigA Promoter ND 247562..247606 CATTTTTCAAAAGGTAACAAATGTTGTATAATAATAGAATTTGAA Cao M & Helmann JD (2004): ROMA RG
SigX Promoter -39:+8 247660..247706 TTCCTGTAACGCTATTCGATCACTATCGTCAAATAATATAGATGGTT Cao M & Helmann JD (2004): ROMA RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AAAAAGAGGAGCTTGCATAAACGCAGCGCCTCTTTTTTTGAAGAAAG
     >>>>>>>>>>>      <<<<<<<<<<
249532..249558
4..30
-13.1
psd

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai