Regulated Operon: thrZ-ywhA

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
thrZ thrS2 - 3853278..3855194 threonyl-tRNA synthetase COG0441 thrZ-BAC  
ywhA
- 3852739..3853158
COG1846  

Operon evidence: Northern blotting (4.4 kb transcript)
Reference: Putzer H, et al. (1992), Presecan E, et al. (1997), Genbank Z80360
Comments: Partial termination at the stem-loop downstream of thrZ gives rise to a 2.5 kb monocistronic thrZ transcript. Northern blotting also showed transcripts of 250 bp, 500 bp, and 750 bp, presumably due to transcriptional termination at the stem-loops in front of thrZ caused by binding of uncharged tRNA-Thr to the T-box sequence motifs. The tRNA anticodon binds to the ACC codon in the UUACCGCC sequences in the thrZ leader mRNA.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter ND 3855997..3856034 CTGATTGCGCTTTGTGGGAATATATAGTAAGATATAAA Putzer H, et al. (1992): NB DP, Western blot
TrnI-Thr Positive ND 3855751..3855814 TGTGTAATTGATCGGGAATTTGGGTGGAACCACGGATGATCAACACATTCGTCCCTTTTAGAGG Putzer H, et al. (1992): RG, Western blot
Gendron N, et al. (1994): OV RG
TrnI-Thr Positive ND 3855496..3855559 CATAGTATTTGTCGGGAACTTGGGTGGAACCACGGGTTAATCACACACTCGTCCCTATCTGTGG Putzer H, et al. (1992): RG, Western blot
TrnI-Thr Positive ND 3855243..3855306 CATAGTATTTGTCGGGAACTTGGGTGGAACCACGGGTTAATCACACACTCGTCCCTATCTGCGG Putzer H, et al. (1992): RG, Western blot

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
ATGATCAACACATTCGTCCCTTTTAGAGGGATGGGTGTGTTTTTTTATTTGAAT
        >>>>>>>>>>>>>>   <<<<<<<<<<<<<<
3855741..3855771
-2493..-2463
-21.2
 thrZ
GGTTAATCACACACTCGTCCCTATCTGTGGGACGGGTGTGTTTTTTTATGAATTG
         >>>>>>>>>>>>       <<<<<<<<<<<<
3855486..3855516
-2238..-2208
-24.1
 thrZ
GGTTAATCACACACTCGTCCCTATCTGCGGGACGGGTGTGTTTTTTTATATACAA
         >>>>>>>>>>>>       <<<<<<<<<<<<
3855233..3855263
-1985..-1955
-24.4
 thrZ
TGAAACAGGTGGAAAATCGAGGCATGTAGGATAAAAGCTGTGAATTCACATTTTGCTTTTTTGAC
        >>>>>>>>>>>>>>>             <<<<<<<<<<<<<
3853258..3853298
-20..20
-12.1
 thrZ
TAAAAGCCGGTGCGCGAAGCGCCGGCTTTATAGAGATTATT
     >>>>>>>>     <<<<<<<<
3852707..3852727
12..32
-18.4
 ywhA

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai