Regulated Operon: tyrS

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
tyrS
- 3035645..3036913 tyrosyl-tRNA synthetase COG0162 tyrS-BAC tyrS-STA tyrS-STR  

Operon evidence: Genome analysis
Reference: Henkin TM, et al. (1992), Grundy FJ & Henkin TM (1993)
Comments: Promoter deletions and reporter gene fusions showed that transcription terminates at the stem-loop upstream of tyrS if charged tyrosine tRNA (TrnD-Tyr) binds to the T-box motif in the nascent mRNA transcript. The tRNA anticodon binds to the UAC codon in the sequence GAAUACACU in the tyrS leader mRNA.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -43:+18 3037193..3037253 AGGCGGCGTTGACACAGGATTTTATTTATGTTAAAAATGATATAGCTTCATATGAAAAGGT Henkin TM, et al. (1992): PE
TrnD-Tyr Positive ND 3036983..3036999 AGTAGGGTGGTACCGCG Henkin TM, et al. (1992): DP RG
Grundy FJ & Henkin TM (1993): RG SDM
Grundy FJ, et al. (1994): SDM RG
Grundy FJ, et al. (1997): SDM RG
Rollins SM, et al. (1997): SDM RG
Grundy FJ, et al. (2000): RG SDM NB
Gerdeman MS, et al. (2002): GS
Grundy FJ, et al. (2002): SDM RG
TrnSL-Tyr1 Positive ND 3036983..3036999 AGTAGGGTGGTACCGCG Henkin TM, et al. (1992): DP RG
Grundy FJ & Henkin TM (1993): RG SDM
Grundy FJ, et al. (1994): SDM RG
Grundy FJ, et al. (1997): SDM RG
Rollins SM, et al. (1997): SDM RG
Grundy FJ, et al. (2000): RG SDM NB
Gerdeman MS, et al. (2002): GS
Grundy FJ, et al. (2002): SDM RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
ATAATCAATCGTCCCTTCGTGTAAACGAAGGGGCGTTTTTTATTTTAATT
         >>>>>>>>>>>    <<<<<<<<<<<
3036948..3036973
-1328..-1303
-22.5
tyrS
AAAAAGATCCTTTGCCACTGAAGGCAAAGGATCTTTTTGTTTACCGCA
     >>>>>>>>>>>      <<<<<<<<<<<
3035611..3035638
7..34
-20.7
tyrS

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai