Regulated Operon: valS-folC

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
valS
- 2865890..2868532 valyl-tRNA synthetase COG0525 valS-BAC valS-STA  
folC
- 2864538..2865830 folyl-polyglutamate synthetase COG0285 folC-STA  

Operon evidence: Northern blotting (4.4 kb transcript)
Reference: Grundy FJ & Henkin TM (1993), Luo D, et al. (1997), Genbank L04520, Genbank X77239
Comments: The readthrough terminator downstream of valS leads to a 3.0 kb monocistronic valS transcript. Based on homology, transcriptional readthrough is expected to occur at the stem-loop upstream of valS if uncharged valine tRNA binds to the T-box motif in the nascent mRNA transcript. The tRNA anticodon binds to the GUC codon in the sequence AAGGUCGCC in the valS leader mRNA.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -43:+8 2868837..2868887 AGATGATTGACGAATCAAAAACACTTTTACTATAATAAAGAACATAAATAA Luo D, et al. (1997): PE NB
TrnSL-Val2 Positive ND 2868601..2868643 GATTGAGTTCATGAAAAAAGGTGGTACCGCGAAAGAGCTTTTC Grundy FJ & Henkin TM (1993): HM
Luo D, et al. (1997): RG SDM OV

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
TACCGCGAAAGAGCTTTTCGTCCTTTTACAGGGATGAAGAGCTCTTTTTTCGTTCTCAG
          >>>>>>>>>>>>>>      <<<<<<<<<<<<<<
2868576..2868609
-2719..-2686
-23.5
valS
GGCTCCGGCAGAATCAAAAAAAGATTCTGCCGTTTTTTTCATGTGTA
     >>>>>>>>>>       <<<<<<<<<<
2864498..2864524
14..40
-18.1
folC
GCCGGGGCGGTTTCTAGAGAAACCGCCTTGTCTTATAAAAGA
     >>>>>>>>>    <<<<<<<<<
2865857..2865878
12..33
-17.1
valS

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai