Regulated Operon: yfhKLM

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
yfhK
+ 928139..928657

 
yfhL
+ 928742..929074

 
yfhM
+ 929061..929921
COG0596  

Operon evidence: Northern blotting (1.8 kb transcript)
Reference: Antelmann H, et al. (2000), Huang X, et al. (1999), Genbank D85082
Comments: Internal promoters in front of yfhL and yfhM, leading to a 1.2 kb and a 0.9 kb transcript, respectively. SubtiList and Genbank D85082 list a terminator after yfhK, but none after yfhM. Northern blotting results in BSORF show a yfhK, a yfhLM, and a yfhKLM transcript. Huang et al. found a sigW-dependent promoter in front of yfhL; the promoter in front of yfhM has not yet been identified.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigB Promoter ND 928069..928111 ACATGTTTCACCAGCCTGTCAATCAGGGAATACCACTTATATC Antelmann H, et al. (2000): HB
SigW Promoter -38:+6 928688..928731 ATGCATGAAACATTTCTTCTTTCTGCACGTAACAATGAGAAAGG Huang X, et al. (1999): S1
Cao M, et al. (2002): AR

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
CATCCGTCTGTCATAATGGCAGACTTTTTCTGTGCGTTT
     >>>>>>>>   <<<<<<<<
929942..929960
21..39
-11.6
yfhM

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai