Regulated Operon: bioWAFDBI-ytbQ

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
bioW
- 3094679..3095455
COG1424H  
bioA
- 3093343..3094689
COG0161H x0337-BAC  
bioF
- 3092184..3093353
COG0156H bioF-BAC  
bioD
- 3091492..3092187
COG0132H  
bioB
- 3090482..3091489
COG0502H  
bioI
- 3089226..3090413
COG2124Q  
ytbQ
- 3088388..3089149
COG0451MG  

Operon evidence: Northern blotting (7.0 kb transcript found by Bower et al.; 7.2 kb transcript found by Perkins et al.).
Reference: Bower S, et al. (1996), Perkins JB, et al. (1996), Genbank AF008220
Comments: The readthrough terminator downstream of bioB leads to a 5.0 kb (as measured by Bower et al.) / 5.1 kb (as measured by Perkins et al.) bioWAFDB transcript. Readthrough termination was confirmed by Perkins et al. by deleting the stem-loop structure downstream of bioB. Perkins et al. also found a monocistronic bioW transcript of 0.8 kb, which may be an RNA degradation product.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
BirA Negative ND 3095505..3095544 CTAATTGTTAACCTTTGAATATAATTGGTTAACAATTTAG Bower S, et al. (1996): DB
Perkins JB, et al. (1996): DB NB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GAACGGAGCATAATCATTTTCTAAGATTATGCTCTTTTTCTTTTGTTAT
     >>>>>>>>>>         <<<<<<<<<<
3088367..3088395
-7..21
-14.9
 ytbQ
AAAAGCAATCGGTATGATGTCGATTGTTTTTATTTTTGAAC
     >>>>>>>>     <<<<<<<<
3090446..3090466
16..36
-7.1
 bioB

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai