Regulated Operon: cggR-gapA-pgk-tpiA-pgm-eno

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
cggR yvbQ - 3482752..3483774
COG2390K  
gapA gap - 3481698..3482705
COG0057G gapA-BAC-1 gapA-MYC-1 gapA-BAC-1 gapA-MYC-1 gapA-MYC-2 stu1788-STA stu1788-STR  
pgk gerG - 3480197..3481381
COG0126G pgk-BAC pgk-CLO pgk-LAB pgk-STA  
tpiA tim - 3479405..3480166
COG0149G tpi-BAC tpi-LAB tpi-STA tpi-STR  
pgm
- 3477877..3479412
COG0696G x0574-STR  
eno
- 3476555..3477847
COG0148G eno-BAC eno-MYC eno-STA eno-STR  

Operon evidence: Northern blotting (7.4 kb transcript)
Reference: Ludwig H, et al. (2001), Genbank L29475
Comments: The readthrough terminator downstream of gapA leads to a 2.2 kb cggR-gapA transcript and a 6.2 kb gapA-pgk-tpiA-pgm-eno transcript. The internal promoters in front of gapA and pgk lead to a 1.2 kb monocistronic gapA transcript and a 4.8 kb pgk-tpiA-pgm-eno transcript.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CggR Negative +27:+72 3483799..3483844 CTATGACGGGACGTTTTTTGTCATAGCGGGACATATAATGTCCAGC Kodama T, et al. (2000): PE RG
Doan T, et al. (2003): DB FT
SigA Promoter -42:+14 3483857..3483912 CTTAACAGTTGAATAAACAATTCCACCCTGTTAAAATAATTAAAGAAAGCAGAAAT Ludwig H, et al. (2001): PE
SigA Promoter -49:+9 3481628..3481685 AAAGGACCTGACTTGGTTCTTTCGAATAGAAGCGCTATAATGAAAGCGGACAAGGGAA Ludwig H, et al. (2001): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAGACCGCCGGGATTTTCTCTCGGCGGCTTTTTTATGCTTTCA
     >>>>>>>>>     <<<<<<<<<
3476524..3476546
9..31
-19.1
 eno
GACAAGGGAAGGGGACGGACTCCCTTTCCCTTTTTCCATGAAGAC
     >>>>>>>>>>     <<<<<<<<<<
3481608..3481632
66..90
-19.4
 gapA

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai