Regulated Operon: drm-punA

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
drm yqkN - 2447246..2448430
COG1015G deoB-STA  
punA pnp, yqkO, yqkO - 2445607..2446422 purine nucleoside phosphorylase
punA-BAC punA-STR pnpA-BAC pnpA-STA pnpA-STR  

Operon evidence: Northern blotting (2.1 kb transcript); transcriptional fusions
Reference: Schuch R, et al. (1999), Wu JJ, et al. (1992)
Comments: Northern blotting results in BSORF show a drm-punA, a ripX-drm-punA, and a fur-ripX-drm-punA transcript. Wu et al. consider a monocistronic punA transcript.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -42:+17 2448467..2448525 AATACATTGTCAACGGGAATGAAAACGGTTTATACTTGAGTTGTCAGACCTCTTAGCGA Schuch R, et al. (1999): PE
SigA Promoter -41:+6 2448449..2448495 TATACTTGAGTTGTCAGACCTCTTAGCGATACAATAGACGTATAGAA Schuch R, et al. (1999): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
CAAAAGACAGCTGTGTCTGATATCACACAGCTGTCTTTTTTTATGCCCAA
     >>>>>>>>>>>        <<<<<<<<<<<
2446364..2446393
-786..-757
-20.2
punA

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai