Regulated Operon: | infC-rpmI-rplT-ysdA |
Genes | Synonyms | Direction | Genome position | Function | COG ID | Conserved groups |
---|---|---|---|---|---|---|
infC | - | 2952828..2953349 | COG0290J | infC-BAC infC-STA infC-STR | ||
rpmI | - | 2952615..2952815 | COG0291J | rpmI-BAC rpmI-STA rpmI-STR | ||
rplT | - | 2952224..2952583 | COG0292J | rplT-MYC rplT-BAC rplT-LAB rplT-MYC rplT-STA rplT-STR | ||
ysdA | - | 2951898..2952167 | COG3326S |
Operon evidence: | Northern blotting (1.7 kb transcript) |
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Reference: | Wipat A, et al. (1996), Choonee N, et al. (2007) |
Comments: | The full-length infC-rpmI-rplT-ysdA transcript is minor; the major transcript has a length of 1.4 kb and includes infC-rpmI-rplT, terminating at the terminator downstream of rplT. Transcriptional readthrough at this terminator was confirmed by RT-PCR experiments. The stem-loop upstream of infC is part of a terminator/antiterminator structure, as shown by Northern blotting, point mutations, lacZ fusions, and in-vitro transcription assays. Binding of the ribosomal protein L20 (RplT) to the nascent mRNA causes transcriptional termination. |
Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
---|---|---|---|---|---|
RplT | Negative | +19:+73 | 2953478..2953532 | AAGCAGAAGCACCCGCTTCTCACCTGATTGACACATGCATGTAGTTGGCAGGTTG |
Choonee N, et al. (2007): NB RG SDM, in-vitro assay, OV FT, toeprint |
SigA | Promoter | -37:+2 | 2953549..2953587 | CTTGACTAAAGATCCGGTATTGTGTAGAATAGTTATTGA |
Choonee N, et al. (2007): PE |
Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
---|---|---|---|---|
ATGTTCGTAGTGTGGGTGTTTTATAATGCCCACACTTTTTGTTTGCCTGC >>>>>>>>>>> <<<<<<<<<<< |
infC | |||
AAAAAGCAGAAAAGCGTTTAACGCTCTTCTGCTTTTTTTGCGAGTTT >>>>>>>>>>>> <<<<<<<<<<<< |
ysdA | |||
ATAGAGCCGCTCTCCAGCAGAGCGGCTTTTTCTATATAAAG >>>>>>>> <<<<<<<< |
rplT |
|