Regulated Operon: lcfA-ysiAB-etfBA

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
lcfA
- 2918646..2920328
COG0318IQ lcfA-BAC  
ysiA
- 2917025..2917609

ysiA-BAC  
ysiB
- 2917166..2917942
COG1024I  
etfB
- 2916378..2917151
COG2086C etfB-BAC x1559-CLO  
etfA
- 2915365..2916342
COG2025C etfA-BAC  

Operon evidence: Northern blotting (5.1 kb transcript)
Reference: Wipat A, et al. (1996), Raposo MP, et al. (2004), Matsuoka H, et al. (2006), Barabesi C, et al. (2007)
Comments: The internal promoter in front of ysiA and read-through terminators downstream of lcfA and ysiA lead to a 1.8 kb monocistronic lcfA transcript, a 0.7 kb monocistronic ysiA transcript, and a 3.2 kb ysiAB-etfBA transcript.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CcpA Negative ND 2919898..2919920 AAAATGAAAACGTTATCAATAGT Miwa Y, et al. (2000): RG
YsiA Negative -43:+24 2918562..2918628 GAATAAACTTGACAAACCTGAAAATCCTTCTTATCATGAAATTATGAATGAATAGTCATTCATTTCT Matsuoka H, et al. (2006): NB DB PE RG FT RG
SigA Promoter -43:+24 2918562..2918628 GAATAAACTTGACAAACCTGAAAATCCTTCTTATCATGAAATTATGAATGAATAGTCATTCATTTCT Matsuoka H, et al. (2006): PE NB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
GAAATCCCGGACTTTAAAAGTCCGGGTTTTTTCATATTTAT
     >>>>>>>>>   <<<<<<<<<
2915337..2915357
8..28
-16.4
etfA

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai