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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai |
| Regulated Operon: | lepA-hemN-hrcA-grpE-dnaKJ-yqeTUV |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Conserved groups |
|---|---|---|---|---|---|---|
| lepA | yqeQ | - | 2630910..2632748 | COG0481M | lp_2015-LAB lp_2015-STA x0099-CLO | |
| hemN | yqeR | - | 2629718..2630857 | COG0635H | x1621-STA | |
| hrcA | yqeS | - | 2628606..2629637 | COG1420K | hrcA-BAC hrcA-STA hrcA-STR | |
| grpE | - | 2627971..2628534 | COG0576O | grpE-STA | ||
| dnaK | - | 2626112..2627947 | COG0443O | dnaK-BAC dnaK-MYC dnaK-STA | ||
| dnaJ | - | 2624785..2625912 | COG0484O | dnaJ-BAC dnaJ-STA dnaJ-STR | ||
| yqeT | - | 2623825..2624760 | COG2264J | yqeT-BAC | ||
| yqeU | - | 2623032..2623802 | COG1385S | |||
| yqeV | - | 2620909..2622264 |
| Operon evidence: | Northern blotting (8.0 kb hrcA-grpE-dnaKJ-yqeTUV transcript and a 7.0 kb lepA-hemN-hrcA-grpE-dnaK transcript) |
|---|---|
| Reference: | Schulz A, et al. (1994), Homuth G, et al. (1996), Hippler B, et al. (1997), Homuth G, et al. (1997), Condon C, et al. (2002), Genbank D83717, Genbank X52064 |
| Comments: | Readthrough terminators downstream of lepA and dnaK lead to a 1.8 kb monocistronic lepA transcript and a 3.6 kb hrcA-grpE-dnaK transcript, respectively. The internal promoter upstream of dnaJ produces to a 4.3 kb dnaJ-yqeTUV transcript. A cleavage site between hrcA and grpE produced a 7.0 kb grpE-dnaKJ-yqeTUV mRNA and a 2.6 kb grpE-dnaK mRNA molecule. Slot-blot and immunoblot experiments with a lepA mutant showed that lepA and hemN are transcribed from the same promoter. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| SigA | Promoter | -58:+12 | 2632774..2632843 | ACTTTTCTCTTTCTTGTTTTACATTGAATCTTTACAATCCTATTGATATAATCTAAGCTAGTGTATTTTG |
Hippler B, et al. (1997): PE |
| ArfM | Positive | ND | ND | ND |
Homuth G, et al. (1999): RG PE |
| HrcA | Negative | +5:+31 | 2629652..2629678 | TTAGCACTCGCTTATTGAGAGTGCTAA |
Zuber U, et al. (1994): SDM HB Voelker U, et al. (1994): 2D PAGE, HB |
| SigA | Promoter | -49:+18 | 2629665..2629731 | TGGGTGAGTTATAATTGACATTTTTCTTGTGGTTTGATACTTTTGTTATAGAATTAGCACTCGCTTA |
Wetzstein M, et al. (1992): PE NB Homuth G, et al. (1997): NB |
| unknown | Promoter | -49:+8 | 2629812..2629868 | CGGCCGTTCCCTGGATGGACTTTTCCCAAGCGTGTTAAAAGACCTCGCTGAAAAAGG |
Wetzstein M, et al. (1992): PE |
| unknown | Promoter | -52:+8 | 2628548..2628607 | AAGGGAATTTTGGCAAATTTTATCGAAGGGCAGCACCTGTCCTTCTCCTTACACTTTGAG |
Wetzstein M, et al. (1992): PE Voelker U, et al. (1994): 2D PAGE |
| SigA | Promoter | ND | 2625933..2625985 | AAAGGAATTGAAAAATCATAATTCAAAATGATACAATCTAATTTATGTGAGAA |
Homuth G, et al. (1997): NB |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| AAAAATCGGTGCATTAAAATGTACCGATTTTTTTATTTAGCC >>>>>>>>> <<<<<<<<< |
yqeV | |||
| AGAAAGTCAAAGTCAGGCATCTCTTGGCTTTGACTTTTTTTCTTGCCCGGG >>>>>>>>>>>> <<<<<<<<<<<< |
dnaK | |||
| TCCGAAAAAACAATAGAAGCCGCCGCAGTCTTGTACTGCGGCTTCTTTCTGTAAAGA >>>>>>>> <<<<<<<< |
lepA |
|