Regulated Operon: menBEC

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
menF entC - 3152302..3153717
COG1169HQ dhbC-BAC  
menD
- 3150563..3152305
COG1165H  
ytxM ytfB - 3149751..3150575
COG0596R  
menB
- 3148901..3149716
COG0447H  
menE
- 3147350..3148810
COG0318IQ  
menC ytfD - 3146238..3147353
COG4948MR  

Operon evidence: Genome analysis
Reference: Lapidus A, et al. (1997), Driscoll JR & Taber HW (1992), Miller P, et al. (1988), Genbank AF008220
Comments: The readthrough terminator downstream of menB was found by RNA protection assays. An S1 nuclease experiment of the 5' end showed that menF and menD belong to the same operon. RNase protection assays showed that transcription of menB occurs from an upstream region in addition to the menB promoter, indicating that either no terminator or a readthrough terminator exists downstream of ytxM.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -45:+18 3153795..3153857 GCTTTGTTTCTTTTTCATTTCTGAAATTAGGTTTATAATAGGTAAGGCAGGCCATTTGGACTG Qin X, et al. (1996): PE
SigA Promoter -47:+17 3149688..3149751 ACTCATTCACATAGAGAATAAAAGGAGGTCATCATATGGCTGAATGGAAAACAAAACGGACATA Driscoll JR, et al. (1992): PE
SigA Promoter -50:+16 3148790..3148855 GCTGTTTTCTTTTCAATACAGACATTTTACCTCGGAGATGATGACATGCTGACAGAACAGCCCAAC Driscoll JR, et al. (1992): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAAAGCATTTTGCTATTTTGAATAAATGAC
     >>>>   <<<<
3146221..3146231
7..17
-5.1
 menC
TTGGACCGGCTGCTGACCCAGCCGGTTTTTTTATGTAAAT
     >>>>>>>      <<<<<<<
3146133..3146152
86..105
-13.4
 menC
ACCAACAGCTTGAGACTTTGCGGTCCAAGCTGTTTTCTTTTCAATAC
     >>>>>>>>>>>>    <<<<<<<<<<<
3148852..3148878
23..49
-17.1
 menB

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai