Regulated Operon: pheST

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
pheS
- 2929438..2930472
COG0016J pheS-BAC pheS-LAB pheS-STA pheS-STR  
pheT
- 2927008..2929422
COG0072J pheS-LAB pheT-BAC pheT-CLO  

Operon evidence: Genome analysis
Reference: Brakhage AA, et al. (1990), Grundy FJ & Henkin TM (1993)
Comments: Transcripts starting about 1.0 kb upstream of the indicated promoter were also detected. These may correspond to a ysgA-pheST transcript. Based on homology, transcriptional readthrough is expected to occur at the stem-loop upstream of pheS if uncharged phenylalanyl tRNA binds to the T-box motif in the nascent mRNA transcript. The tRNA anticodon binds to the UUC codon in the sequence GCCUUCACC in the pheS leader mRNA.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -38:+5 2930786..2930828 AGTGTTGCGCCAAGCGTTGGATTTCTCTATAATAGAACATAAT Brakhage AA, et al. (1990): PE S1
TrnB-Phe Positive ND 2930551..2930589 AGTCTGTCTGAAATAAGGGTGGTACCGCGGCCACAACTC Grundy FG & Henkin TM (1993): HM
TrnD-Phe Positive ND 2930551..2930589 AGTCTGTCTGAAATAAGGGTGGTACCGCGGCCACAACTC Grundy FG & Henkin TM (1993): HM

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
TACCGCGGCCACAACTCGTCCCTTGTACAAGGGACGGGTTTTTTTTATTTTCC
              >>>>>>>>>>    <<<<<<<<<<
2930530..2930553
-1115..-1092
-17.8
 pheS
TTAAGAGGCTGATAAAAAAGCTTGCAGAGAAATCTGCAAGCTTTTTTCTATGAACG
                   >>>>>>>>>    <<<<<<<<<
2926979..2927000
8..29
-18.8
 pheT

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai