Regulated Operon: rapG-phrG

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
rapG yycM + 4140260..4141357

 
phrG yycL + 4141358..4141474

 

Operon evidence: Northern blotting (1.6 kb transcript); primer extension of phrG
Reference: McQuade RS, et al. (2001), Ogura M, et al. (2003), BSORF
Comments: Internal promoter in front of phrG leads to monocistronic phrG transcripts of 0.6 kb and 0.4 kb.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
YvaN Negative -55:-27 4140160..4140188 CTTCATATATTGACTAGAGGATTTTACAT Hayashi K, et al. (2006): RG DB AR PE GS FT
SigA Promoter -34:+5 4140181..4140219 TTTTACATAAAATAGAAAGAGGTGTTACTATCAGAATAA Hayashi K, et al. (2006): PE
SigH Promoter -38:+4 4141080..4141121 TGAAGGAAAAATTGACTGCTGAGCCGAATAGAATATGTGAGG McQuade RS, et al. (2001): PE RG DB
Ogura M, et al. (2003): DB NB RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AAGAATCCTAAAACGGTTTGTAGTTTTAGGATTCTTTCATCTTTTC
     >>>>>>>>>        <<<<<<<<<
4141520..4141545
46..71
-12.9
phrG

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai