Regulated Operon: sacPA-ywdA

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
sacP ipa-49d - 3903646..3905031
COG1263G scrA-BAC  
sacA ipa-50d - 3902210..3903649
COG1621G  
ywdA ipa-51d - 3901868..3902116

 

Operon evidence: Genome analysis
Reference: Fouet A, et al. (1986), Debarbouille M, et al. (1990), Arnaud M, et al. (1992), Aymerich S & Steinmetz M (1992)
Comments: In-vitro transcription experiments showed that transcription terminates at the stem-loop structure upstream of sacP; transcriptional termination is alleviated in the presence of sucrose by SacT (and to a lesser degree SacY) binding to the ribonucleic antiterminator (RAT) sequence.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SacT Positive +21:+63 3905117..3905159 ATAAGCGGGATTGTGACTGGTAAAGCAGGCAAGACCTAAAATT Lepesant JA, et al. (1976): Microbiology-1976, edited by David Schlessinger; pages 58-69: DB, sucrase activity
Debarbouille M, et al. (1990): DB HM, sucrase activity
Arnaud M, et al. (1992): SDM RG DB
Arnaud M, et al. (1996): GS, in-vitro transcription
SacY Positive +21:+63 3905117..3905159 ATAAGCGGGATTGTGACTGGTAAAGCAGGCAAGACCTAAAATT Lepesant JA, et al. (1976): Microbiology-1976, edited by David Schlessinger; pages 58-69: DB, sucrase activity
Arnaud M, et al. (1996): GS, in-vitro transcription
SigA Promoter -38:+13 3905167..3905217 AAAAATAGTTGACGAAAACGCTATCATGATTTATGATGAAAGCGTATTCTT Arnaud M, et al. (1996): RO

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
CAAGACCTAAAATTTGCGTAAATGAAAAAGGATCGCTGTGTCCTTTATTCGTTGGCGAATTTTAGGTCTTTTTT
            >>>>>>>>>>>>>>>>>>>>>      <<<<<<<<<<<<<<<<<<<<
3905072..3905118
-1472..-1426
-18.8
 sacP
ACAGGCTGCCGATGGGATCGGCAGTTTTTTCTGTGAAAA
     >>>>>>>>   <<<<<<<<
3901844..3901862
6..24
-13.4
 ywdA

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai