Regulated Operon: thrS

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
thrS
- 2959257..2961188
COG0441J thrS-BAC thrS-LAB thrS-STA thrZ-BAC  

Operon evidence: Northern blotting (2.3 kb transcript)
Reference: Putzer H, et al. (1992), Grundy FJ & Henkin TM (1993), Wipat A, et al. (1996)
Comments: Runoff transcription, Northern blotting, and S1 nuclease mapping also showed a 280 bp transcript, presumably due to transcriptional termination at the stem-loop in front of thrS caused by binding of uncharged tRNA-Thr to the T-box sequence motif. The tRNA anticodon binds to the ACC codon in the sequence UUUACCGCC in the thrS leader mRNA.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -42:+20 2961475..2961536 GCGATCGTGTTGATTTTTTGGATTGAACAATTTATAATACATAGGAGATTAAGAAAGACACA Gendron N, et al. (1994): PE RO
TrnI-Thr Positive ND 2961251..2961285 TTTGCGGAAAAAAGGGTGGAACCACGATTCCGTTT Putzer H, et al. (1992): RG SDM, Western blot
Gendron N, et al. (1994): RO OV RG DP NB
Putzer H, et al. (1995): DP RG SDM
Putzer H, et al. (2002): RO RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
ATTCCGTTTATTCAACCTCGTCCCTTTCATAGGGGGCGGGGTTTTTATATGCAAAA
               >>>>>>>>>>      <<<<<<<<<<
2961219..2961244
-1987..-1962
-19.3
thrS
AAAAAGCATGATCTCATTGAAGAGATCATGCTTTTTTTATTTCTCT
     >>>>>>>>>>      <<<<<<<<<<
2959222..2959247
10..35
-17.9
thrS

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai