Procedures
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1. Setup python |
Hon-yaku is a python script.
Please install Python and Python modules (Numerical Python and statistics) following linked pages.
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2. Construct your true dataset |
After installing python and the modules, you need to make the true dataset which contains homologos sequences with the EcoGene dataset or the B. subtilis dataset.
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3. Align ribosome binding motif |
We recommend MEME motif finding program to align the ribosomal factor binding motifs.
We also recommend to use Se-Al (after running MEME) for human curation.
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4. Setup the RBS motif, and so on. |
We provide several short scripts for making the RBS motif weight matix, and so on.
Details are written in the README file of our package. |
5. Run the script |
You can download Hon-yaku from the Download page.
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