Regulated Operon: tenAI-goxB-thiSGF-yjbV

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
tenA
+ 1241761..1242471 transcriptional regulator COG0819 tenA-BAC x0362-STA  
tenI
+ 1242446..1243063 transcriptional regulator COG0352  
goxB yjbR + 1243047..1244156 glycine oxidase COG0665  
thiS yjbS + 1244156..1244356
COG2104 x0478-BAC  
thiG yjbT + 1244353..1245123
COG2022  
thiF yjbU + 1245120..1246130
COG0476  
yjbV
+ 1246149..1246964
COG0351 yjbV-BAC  

Operon evidence: lacZ transcriptional fusions; genome analysis
Reference: Pang AS, et al. (1991), Mironov AS, et al. (2002)
Comments: The lacZ transcriptional fusions showed that tenA and tenI belong to the same operon. Genes further downstream are assigned to this operon based on genome analysis. A terminator-antiterminator structure is found upstream of tenA. Binding of thiamin pyrophosphate to the thi-box favors transcriptional termination.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
TenI Negative ND ND ND Pang AS, et al. (1991): DB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
GTTATTTTACTATGCCAATTCCAAACCACTTTTCCTTGCGGGAAAGTGGTTTTTTTATTTTCAG
                         >>>>>>>>>>    <<<<<<<<<<
1241708..1241731
-763..-740
-15.6
tenA
TTAAATACAAGCCGATGAGATCACCAGCTGATGGTGATCTCTTTTGCGTTTGCTAC
                >>>>>>>>>>     <<<<<<<<<<
1246963..1246987
-1..23
-18.5
yjbV

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai