Regulated Operon: tyrZ

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
tyrZ ipa-9r, tyrS1, tyrT + 3946179..3947420 tyrosyl-tRNA synthetase COG0162  

Operon evidence: Genome analysis
Reference: Henkin TM, et al. (1992), Presecan E, et al. (1997)
Comments: Transcriptional readthrough is presumed to occur at the stem-loop upstream of tyrZ if uncharged tyrosine tRNA (TrnD-Tyr) binds to the T-box motif in the nascent mRNA transcript. The tRNA anticodon binds to the UAC codon in the sequence AAUUACCUC in the tyrZ leader mRNA.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
TrnD-Tyr Positive ND 3946099..3946115 ACCAGGGTGGTACCGCG Henkin TM, et al. (1992): HM
TrnSL-Tyr1 Positive ND 3946099..3946115 ACCAGGGTGGTACCGCG Henkin TM, et al. (1992): HM

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
TACCGCGTGCATTGAGCCACGTCCCTTATTGGGATGGGCTCTTTTTTGTGTTTGTA
             >>>>>>>>>>>>     <<<<<<<<<<<
3946122..3946149
-1298..-1271
-20.1
 tyrZ
CGAAACGCTTATGACCCTTCATTCATAAGCGTTTTTTTGCAGGTAT
     >>>>>>>>>        <<<<<<<<<
3947436..3947461
16..41
-12.9
 tyrZ

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai