Regulated Operon: pdhABCD

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
pdhA aceA + 1528326..1529441
COG1071C pdhA-BAC pdhA-STA aceA-BAC  
pdhB
+ 1529445..1530422
COG0022C  
pdhC
+ 1530537..1531865
COG0508C pdhC-BAC  
pdhD citL + 1531870..1533282
COG1249C  

Operon evidence: Northern blotting (5.2 kb transcript)
Reference: Hemila H, et al. (1990), Gao H, et al. (2002)
Comments: Hemila also found a 2.8 kb pdhCD and a 1.7 kb monocistronic transcript. However, Gao et al. note that promoters were found in front of pdhA and pdhC only; no promoter was found in front of pdhD.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -64:+12 1528191..1528266 AAGGTTTTGGCAAACTGGGGATCGGCTAAAATATAATACGACTTACTGCTGATACTTTAGGGTACCCTAAGTTTGT Gao H, et al. (2002): NB PE
Hemila H, et al. (1990): NB
SigA Promoter -70:+8 1530383..1530460 AGACGTTCTTGAAACAGCAAGAAAAGTGCTTGAATTTTAATCAAACTGCATAATCGAGAGGGAAGATGAACGTTTTCC Gao H, et al. (2002): NB PE
Hemila H, et al. (1990): NB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAACAGCCCCGCTTTGAGCGAGGGCTGTTTTTTTATTTTGAC
     >>>>>>>>>>   <<<<<<<<<<<
1533298..1533321
16..39
-18.5
 pdhD

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai