Regulated Operon: trpEDCFBA-hisC-tyrA-aroE

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
trpE
- 2375869..2377416
COG0147EH x1522-STA  
trpD
- 2374881..2375897
COG0547E  
trpC
- 2374139..2374888
COG0134E  
trpF
- 2373487..2374134
COG0135E  
trpB
- 2372304..2373506
COG0133E  
trpA
- 2371508..2372311
COG0159E  
hisC aroJ - 2370415..2371497
COG0079E x1197-BAC x1361-STA x1594-BAC  
tyrA
- 2369251..2370366
COG0287E tyrA-STA  
aroE
- 2367954..2369240
COG0128E aroE-BAC aroE-STA  

Operon evidence: integrative plasmids to interrupt transcription
Reference: Shimotsu H & Henner DJ (1984), Henner DJ, et al. (1985), Henner DJ, et al. (1986), Shimotsu H, et al. (1986), Babitzke P & Gollnick P (2001)
Comments: Transcription starting from the promoter in front of aroF leads to an aroFBH-trpEDCFVA-hisC-tyrA-aroE transcript and an aroFBH transcript, terminating at the terminator/antiterminator structure in front of trpE, as shown by Northern blotting, 3' S1 nuclease mapping, promoter deletions, site-directed mutagenesis, and reporter gene fusions. Readthrough transcription takes place under tryptophan limitation. The MtrB product (TRAP) causes both transcriptional and translational attenuation. In case of transcriptional attenuation, termination occurs at the GT in the T-stretch TTTATTTGT. NusA stimulates transcriptional pausing and termination. Translational attenuation may be followed by transcriptional attenuation further downstream: The absence of the ribosome allows access of the Rho protein to the nascent mRNA transcript, resulting in Rho-dependent transcriptional termination. An internal promoter may exist upstream of hisC.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
Rho Negative ND ND ND Yakhnin H, et al. (2001): DB RG HB
SigA Promoter -47:+8 2377612..2377666 CCGATTTATCATTGACAAAAAATACTGAATTGTAATACGATAAGAACAGCTTAGA Shimotsu H & Henner DJ (1984): S1 DP RG
Shimotsu H, et al. (1986): S1
MtrB Negative ND 2377529..2377584 TAGAATGAGTTGAGTTAGAGAATAGGGTAGCAGAGAATGAGTTTAGTTGAGCTGAG Steinberg W (1974): anthranilate and tryptophan synthase measurement
Kane JF (1977): DB, tryptophan excretion measurement
Shimotsu H & Henner DJ (1984): DP
Shimotsu H, et al. (1986): NB DP SDM RG
Kuroda MI, et al. (1988): DP RG
Gollnick P, et al. (1990): DB RG OV
Babitzke P, et al. (1992): RG OV
Otridge J & Gollnick P (1993): RO NB GS
Babitzke P & Yanofsky C (1993): RO NB OV GS DP
Babitzke P, et al. (1994): filter binding assay, FT
Antson AA, et al. (1995): DP RG SDM
Merino E, et al. (1995): RG SDM DP
Lee AI, et al. (1996): DB RG DP
Yang M, et al. (1997): RG GS DB
Du H & Babitzke P (1998): RG DB, in-vitro translation, ribosome toeprint, RNA structure mapping
Sudershana S, et al. (1999): DP RG SDM DB GS OV NB, in-vitro transcription
Du H, et al. (2000): GS FT RG DP
Valbuzzi A & Yanofsky C (2001): DB RG
Yakhnin AV & Babitzke P (2002): RO

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
CATTATGTTTATTCTACCCAAAAGAAGTCTTTCTTTTGGGTTTATTTGTTATATA
                >>>>>>>>>>    <<<<<<<<<<
2377488..2377511
-1642..-1619
-14.9
trpE
AAAAATCCTGAAGTTTTACTTCAGGATTTTTTATGCAGATC
     >>>>>>>>>   <<<<<<<<<
2367939..2367959
-5..15
-14.6
aroE

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai