Regulated Operon: tyrZ

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
tyrZ tyrT + 3947158..3948399
COG0162J  

Operon evidence: Genome analysis
Reference: Henkin TM, et al. (1992), Presecan E, et al. (1997)
Comments: Transcriptional readthrough is presumed to occur at the stem-loop upstream of tyrZ if uncharged tyrosine tRNA (TrnD-Tyr) binds to the T-box motif in the nascent mRNA transcript. The tRNA anticodon binds to the UAC codon in the sequence AAUUACCUC in the tyrZ leader mRNA.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
TrnD-Tyr Positive ND 3947078..3947094 ACCAGGGTGGTACCGCG Henkin TM, et al. (1992): HM
TrnSL-Tyr1 Positive ND 3947078..3947094 ACCAGGGTGGTACCGCG Henkin TM, et al. (1992): HM

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
TACCGCGTGCATTGAGCCACGTCCCTTATTGGGATGGGCTCTTTTTTGTGTTTGTA
             >>>>>>>>>>>>     <<<<<<<<<<<
3947101..3947128
-1298..-1271
-20.1
 tyrZ
CGAAACGCTTATGACCCTTCATTCATAAGCGTTTTTTTGCAGGTAT
     >>>>>>>>>        <<<<<<<<<
3948415..3948440
16..41
-12.9
 tyrZ

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai