Regulated Operon: araABDLMNPQ-abfA

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
araA
- 2947385..2948875
COG2160G  
araB
- 2945689..2947371
COG1069C  
araD
- 2944983..2945672
COG0235G araD-BAC  
araL yseA - 2944187..2945005
COG0647G  
araM yseB - 2943006..2944190
COG0371C  
araN yseC - 2941674..2942975
COG1653G  
araP yseD - 2940697..2941638
COG1175G  
araQ yseE - 2939851..2940696
COG0395G  
abfA
- 2938330..2939832
COG3534G  

Operon evidence: Northern blotting (11.0 kb transcript)
Reference: Sa Nogueira I & Ramos SS (1997), Genbank X89810
Comments: premature transcription termination or internal promoters may have lead to other detected mRNA lengths. One more putative terminator is listed in the Genbank record.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
AraR Negative -8:+17 2948956..2948980 ATAAAATTGTTCGTACAAATATTTA Sa Nogueira & Mota LJ (1997): DB HM
Mota LJ, et al. (1999): FT
AraR Negative +38:+58 2948915..2948935 CATTAGTACGTATCTTTTGTA Sa Nogueira I & Mota LJ (1997): DB HM
Mota LJ, et al. (1999): FT
CcpA Negative +50:+83 2948889..2948922 CTTTTGTATTTGAAAGCGTTTTATTTTATGAGAA Inacio JM, et al. (2003): RG NB
SigA Promoter -50:+10 2948963..2949012 AAAGATTGACAGTATAATAGTCAATTACTATAATAAAATTGTTCGTACAA Sa Nogueira I & Ramos SS (1997): PE HM
CcpA Negative ND 2946674..2946696 TCAATGAAAACGATTACAAAGGA Miwa Y, et al. (2000): RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
GCAAAGCCGGAGATTTCTCTCCGGCTTGTCTTTCAACTGC
     >>>>>>>>    <<<<<<<<
2938300..2938319
11..30
-16.4
abfA

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai