Regulated Operon: bglPH-yxiE

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
bglP sytA - 4033778..4035607
COG1263G  
bglH
- 4032346..4033755
COG2723G  
yxiE
- 4031797..4032243
COG0589T  

Operon evidence: Northern blotting (4.0 kb transcript)
Reference: Le Coq D, et al. (1995), Kruger S & Hecker M (1995), Kruger S, et al. (1996), Yoshida K, et al. (2000), Antelmann H, et al. (2000)
Comments: Internal promoter in front of yxiE, leading to a 0.5 kb transcript. In the presence of beta-glucosides, LicT binds to the ribonucleic antiterminator (RAT) sequence just upstream of the transcriptional terminator downstream of the bglP promoter, alleviating termination at this stem-loop.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CcpA Negative -47:+3 4035814..4035863 AAAATGAAAGCGTTGACATCTCACGAATCTAGTGCTAAAGTATACCTACA Kruger S, et al. (1996): RO DP
LicT Positive +25:+57 4035760..4035792 GGATTGTTACTGCGAAAGCAGGCAAAACCTAAA Kruger S & Hecker M (1995): RG DB
Kruger S, et al. (1996): RO DP
SigA Promoter -47:+3 4035814..4035863 AAAATGAAAGCGTTGACATCTCACGAATCTAGTGCTAAAGTATACCTACA Le Coq D, et al. (1995): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AAAAAGCGCCCTTGGGCGCTTTTTTTTATTTAGA
     >>>>>    <<<<<
4031770..4031783
14..27
-11.9
yxiE
CCTAAATGGTGCAGGTGCAGGAGGAGACTCTCGCAATCTGTGTCATTTAGGTTTTTTTAT
      >>>>>>>>>>>>>>>>>    <<<<<<<<<<<<<<<<<<
4035721..4035759
-1981..-1943
-20.6
bglP

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai