Regulated Operon: dnaAN

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
dnaA dnaK + 410..1750
COG0593L dnaA-BAC dnaA-STA dnaA-STR dnaK-BAC dnaK-MYC dnaK-STA  
dnaN dnaK + 1939..3075
COG0592L dnaN-BAC dnaN-STA dnaN-STR x1222-CLO dnaK-BAC dnaK-MYC dnaK-STA  

Operon evidence: Northern blotting (2.9 kb transcript); transcriptional experiments
Reference: Ogura Y, et al. (2001), Moriya S, et al. (1988), Moriya S, et al. (1985), Ogasawara N, et al. (1985), BSORF
Comments: Readthrough terminator downstream of dnaA, leading to a 1.7 kb transcript. The earlier result (Ogasawara et al., 1985) showing an internal promoter in front of dnaN is incorrect (Ogura et al., 2001; Ogasawara, personal communication). The Northern blotting experiments listed in BSORF suggest that readthrough may occur at the terminator downstream of dnaN.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
DnaA Negative -170:-147 4215593..10 TCACGCATGTGGATATCTTTTTCG Fukuoka T, et al. (1990): FT (medium)
DnaA Negative -146:-120 11..37 GCTTTTTTTAGTATCCACAGAGGTTAT Fukuoka T, et al. (1990): FT (medium)
DnaA Negative -133:-107 24..50 TCCACAGAGGTTATCGACAACATTTTC Fukuoka T, et al. (1990): FT (medium)
DnaA Negative -99:-80 58..77 CAACCCCTGTGGACAAGGTT Fukuoka T, et al. (1990): FT (medium)
DnaA Negative -64:-36 93..121 CCGCTTTGTGGATAAGATTGTGACAACCA Fukuoka T, et al. (1990): FT (strong)
DnaA Negative +30:+63 186..219 TTCCTCTTTATCCACAAAGTGTGGATAAGTTGTG Fukuoka T, et al. (1990): FT (strong)
DnaA Negative +80:+97 246..263 AGGTTGTCCACAAGTTGT Fukuoka T, et al. (1990): FT (medium)
DnaA Negative between dnaA and dnaN 1767..1837 TCGGGGAAAGTGTGAATAACTTTTCGGAAGTCATACACAGTCTGTCCACATGTGGATAGGCTGTGTTTCCT Fukuoka T, et al. (1990): FT (medium)
DnaA Negative between dnaA and dnaN 1843..1871 TTTCACAACTTATCCACAAATCCACAGGC Fukuoka T, et al. (1990): FT (strong)
SigA Promoter -47:+18 110..174 TTGTGACAACCATTGCAAGCTCTCGTTTATTTTGGTATTATATTTGTGTTTTAACTCTTGATTAC Moriya S, et al. (1988): S1
Ogasawara N, et al. (1985): S1
Spo0A Negative ND ND ND Molle V, et al. (2003): GS CH

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
ACACTGCTGCCGACCCGTCGGCAGCTTTTCTATTCGGTAT
     >>>>>>>>    <<<<<<<<
3087..3106
12..31
-16.0
dnaN

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai