Regulated Operon: gltX-cysES-yazC-yacOP

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
gltX
+ 111047..112498
COG0008J gltX-BAC gltX-STA gltX-STR  
cysE cysA + 112800..113453
COG1045E cysE-BAC-1 cysE-BAC-2 cysE-STA  
cysS snpA + 113450..114850
COG0215J  
yazC
+ 114851..115282

 
yacO
+ 115266..116015

 
yacP
+ 116025..116537
COG3688R  

Operon evidence: Primer extension analysis of cysS; Northern blotting; S1 nuclease mapping
Reference: Gagnon Y, et al. (1994), Pelchat M & Lapointe J (1999), BSORF
Comments: Northern blotting of gltX shows a 1.7 kb transcript. The gltX-cysES-yazC-yacOP transcript is rapidly processed to generate a gltX and a cysES-yazC-yacOP mRNA molecule. RNA cleavage occurs between the last C of the stem-loop and the first T of the T-stretch of the terminator downstream of gltX. Transcriptional readthrough is expected to occur at this stem-loop if uncharged cysteine tRNA binds to the T-box motif in the nascent mRNA transcript. The tRNA anticodon binds to the UGC codon in the sequence AAGUGCGCC in the cysE leader mRNA. Northern blotting results in BSORF show a gltX, a gltX-cysES-yazC-yacOP, and a cysES-yazC-yacOP transcript.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -52:+11 110951..111013 GGCTAACTTGTTTTGATGCCGGGTCTATTTGGTGGTAGAATAGATTCATACATTTTTGCCTGA Gagnon Y, et al. (1994): S1
TrnD-Cys Positive ND 112689..112721 CTTTTCAAACAGAGTGGAACCGCGCGGTTAAAG Gagnon Y, et al. (1994): HM NB OV RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
TTAAAGCGTCTCTGTCATGTTTACATGCAGAGACGCTTTTTTTATTGGGTA
     >>>>>>>>>>>>>>    <<<<<<<<<<<<<
112721..112751
223..253
-23.9
 gltX

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai