Regulated Operon: hut

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
hutP hutP1 + 4041483..4041938

hutP-BAC  
hutH
+ 4042051..4043577
COG2986E hutH-BAC  
hutU
+ 4043574..4045232
COG2987E hutU-BAC  
hutI
+ 4045245..4046510
COG1228Q hutI-BAC x1372-BAC  
hutG
+ 4046503..4047462
COG0010E x0909-STA  
hutM
+ 4047538..4048965
COG0833E  

Operon evidence: Northern blotting (8.0 kb transcript); 5' primer extension analysis of hutP, hutH, and hutU
Reference: Yoshida K, et al. (1995), Yoshida K, et al. (2000), Oda M, et al. (2004), Oda M, et al. (1988), Wray LV Jr & Fisher SH (1994), Oda M, et al. (2000)
Comments: The stem-loop downstream of hutP is part of a terminator/antiterminator structure mediated by HutP, leading to a 0.6 kb transcript.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
AbrB Positive +199:+221 4041649..4041671 CAATTGAAACCGCTTCCAAAAAG Fisher SH, et al. (1994): FT
CcpA Negative +2:+15 4041452..4041465 GTTAATAGTTATCA Wray LV Jr, et al. (1994): SDM DP
CcpA Negative +203:+216 4041653..4041666 TGAAACCGCTTCCA Wray LV Jr, et al. (1994): SDM DP
CodY Negative -3:+23 4041448..4041473 CTCAGTTAATAGTTATCAGAATTTTT Fisher SH, et al. (1996): DB DP
SigA Promoter -46:+17 4041405..4041467 AAAAAACCTTTTGACTTCTGCTGCTGAACCAATTAATATAATACTCAGTTAATAGTTATCAGA Wray LV Jr, et al. (1994): PE
HutP Positive Between hutP and hutH 4041959..4042010 CGATAGGGGGCTATGCGTGAAAACAGAAGTTCACAGCATAGCTCCTTTTTGT Wray LV Jr, et al. (1994): RO SDM
Oda M, et al. (2000): GS

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAAGCACACCCCGCTCAGCATGGGATGTGCTTTATTTTTTATTCT
     >>>>>>>>>         <<<<<<<<<
4048974..4049000
9..35
-13.7
 hutM
GATAGGGGGCTATGCGTGAAAACAGAAGTTCACAGCATAGCTCCTTTTTGTATGGGCGC
     >>>>>>>>>>>>>>>        <<<<<<<<<<<<<<<<
4041965..4042003
27..65
-25.3
 hutP

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai