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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai |
| Regulated Operon: | ilvBHC-leuABCD |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Conserved groups |
|---|---|---|---|---|---|---|
| ilvB | - | 2895248..2896972 | COG0028EH | ilvB-2-BAC ilvB-BAC ilvB-STA | ||
| ilvH | ilvN | - | 2894733..2895251 | COG0440E | ||
| ilvC | - | 2893681..2894709 | COG0059EH | ilvC-BAC ilvC-STA ilvC-STR | ||
| leuA | - | 2892138..2893694 | COG0119E | leuA-STA | ||
| leuB | leuC | - | 2891020..2892117 | COG0473CE | leuB-BAC leuC-STA | |
| leuC | - | 2889552..2890970 | COG0065E | leuC-STA | ||
| leuD | - | 2888940..2889539 | COG0066E |
| Operon evidence: | Northern blotting (8.5 kb transcript) |
|---|---|
| Reference: | Grandoni JA, et al. (1992), Grandoni JA, et al. (1993), Mader U, et al. (2004) |
| Comments: | RNA processing leads to a 5.8 kb ilvC-leuABCD transcript. Runoff transcription, primer extension, promoter deletions, reporter gene fusions, and site-directed mutagenesis showed that readthrough occurs at the terminator in the ilvB promoter when uncharged tRNA-Leu binds to the T-box sequence motif in the nascent tRNA transcript. The tRNA anticodon binds to the CUC codon in the sequence GAACUCGCC in the ilvB leader mRNA. The stem-loop structure in the 5' region of leuA may be a readthrough terminator or a mRNA cleavage site, leading to a monocistronic 1.2 kb ilvC transcript. The second stem-loop shown downstream of leuA is an alternative secondary structure in this region that is followed by a T-stretch, suggesting a readthrough terminator. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| CcpA | Positive | -106:-53 | 2897507..2897560 | TGTACCAATAATGAAAGCGTATACAATATAGATTGATTAATCAAAATTGTCTAA |
Tojo S, et al. (2005): FT Shivers RP, et al. (2005): FT |
| CodY | Negative | -199:+1 | 2897454..2897653 | TCTTCCATGTTTATATAAAATTAAATAATTCTGGTGTATTCTTGATAGATTAATAAAAAAATTATAAAAACTTTTAACATTTGCAATTCCTTTTGTACCAATAATGAAAGCGTATACAATATAGATTGATTAATCAAAATTGTCTAATAATTTTAAAAAATGCTGTTGACACTGCGTCCAAAGCGGCGTAATATGAGTTC |
Molle V, et al. (2003): CH RG GS Mader U, et al. (2004): NB Shivers RP, et al. (2004): FT |
| SigA | Promoter | -45:+19 | 2897436..2897499 | AAAAAATGCTGTTGACACTGCGTCCAAAGCGGCGTAATATGAGTTCAACAAAAGATAAATGCAA |
Grandoni JA, et al. (1992): PE SDM RG |
| TnrA | Negative | -210:-193 | 2897632..2897681 | CATATACGAACCATATCGTGTTATAAAATCTTCCATGTTTATATAAAATT |
Tojo S, et al. (2004): FT |
| TrnS-Leu2 | Positive | ND | 2897120..2897138 | AAACAAGGGTGGTACCGCG |
Grandoni JA, et al. (1993): DP RG PE SDM, in-vitro transcription Garrity DB & Zahler SA (1994): DB RG |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| TTTTCGCCCCTTTTAGCTATCGCAGTTACTGCGCGGCTGATTGTGGGCGGAAGGGCTTTTTTTATTGAATA >>>>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<<<< |
ilvB | |||
| CATCATTGAAGCGGGATTTCCCGCTTCGTCCCGAGGTGACTTTTTAGCTGTTCAGGA >>>>>>>>> <<<<<<<<< |
leuA | |||
| TTTCCCGCTTCGTCCCGAGGTGACTTTTTAGCTGTTCAG >>>>>>> <<<<<<< |
leuA | |||
| AAAAAGCGGCCGGTATTTGTCCGGCGGCTTTTTTTGCCTGGTG >>>>>>>>> <<<<<<<<<< |
leuD |
|