Regulated Operon: ilvBHC-leuABCD

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
ilvB
- 2895248..2896972
COG0028EH ilvB-2-BAC ilvB-BAC ilvB-STA  
ilvH ilvN - 2894733..2895251
COG0440E  
ilvC
- 2893681..2894709
COG0059EH ilvC-BAC ilvC-STA ilvC-STR  
leuA
- 2892138..2893694
COG0119E leuA-STA  
leuB leuC - 2891020..2892117
COG0473CE leuB-BAC leuC-STA  
leuC
- 2889552..2890970
COG0065E leuC-STA  
leuD
- 2888940..2889539
COG0066E  

Operon evidence: Northern blotting (8.5 kb transcript)
Reference: Grandoni JA, et al. (1992), Grandoni JA, et al. (1993), Mader U, et al. (2004)
Comments: RNA processing leads to a 5.8 kb ilvC-leuABCD transcript. Runoff transcription, primer extension, promoter deletions, reporter gene fusions, and site-directed mutagenesis showed that readthrough occurs at the terminator in the ilvB promoter when uncharged tRNA-Leu binds to the T-box sequence motif in the nascent tRNA transcript. The tRNA anticodon binds to the CUC codon in the sequence GAACUCGCC in the ilvB leader mRNA. The stem-loop structure in the 5' region of leuA may be a readthrough terminator or a mRNA cleavage site, leading to a monocistronic 1.2 kb ilvC transcript. The second stem-loop shown downstream of leuA is an alternative secondary structure in this region that is followed by a T-stretch, suggesting a readthrough terminator.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CcpA Positive -106:-53 2897507..2897560 TGTACCAATAATGAAAGCGTATACAATATAGATTGATTAATCAAAATTGTCTAA Tojo S, et al. (2005): FT
Shivers RP, et al. (2005): FT
CodY Negative -199:+1 2897454..2897653 TCTTCCATGTTTATATAAAATTAAATAATTCTGGTGTATTCTTGATAGATTAATAAAAAAATTATAAAAACTTTTAACATTTGCAATTCCTTTTGTACCAATAATGAAAGCGTATACAATATAGATTGATTAATCAAAATTGTCTAATAATTTTAAAAAATGCTGTTGACACTGCGTCCAAAGCGGCGTAATATGAGTTC Molle V, et al. (2003): CH RG GS
Mader U, et al. (2004): NB
Shivers RP, et al. (2004): FT
SigA Promoter -45:+19 2897436..2897499 AAAAAATGCTGTTGACACTGCGTCCAAAGCGGCGTAATATGAGTTCAACAAAAGATAAATGCAA Grandoni JA, et al. (1992): PE SDM RG
TnrA Negative -210:-193 2897632..2897681 CATATACGAACCATATCGTGTTATAAAATCTTCCATGTTTATATAAAATT Tojo S, et al. (2004): FT
TrnS-Leu2 Positive ND 2897120..2897138 AAACAAGGGTGGTACCGCG Grandoni JA, et al. (1993): DP RG PE SDM, in-vitro transcription
Garrity DB & Zahler SA (1994): DB RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
TTTTCGCCCCTTTTAGCTATCGCAGTTACTGCGCGGCTGATTGTGGGCGGAAGGGCTTTTTTTATTGAATA
       >>>>>>>>>>>>>>>>>>>>>>>    <<<<<<<<<<<<<<<<<<<<<
2897053..2897100
-1852..-1805
-20.0
ilvB
CATCATTGAAGCGGGATTTCCCGCTTCGTCCCGAGGTGACTTTTTAGCTGTTCAGGA
      >>>>>>>>>    <<<<<<<<<
2893548..2893569
-1431..-1410
-16.0
leuA
TTTCCCGCTTCGTCCCGAGGTGACTTTTTAGCTGTTCAG
     >>>>>>>   <<<<<<<
2893538..2893554
-1416..-1400
-9.1
leuA
AAAAAGCGGCCGGTATTTGTCCGGCGGCTTTTTTTGCCTGGTG
     >>>>>>>>>    <<<<<<<<<<
2888913..2888935
5..27
-13.5
leuD

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai