Regulated Operon: mmsA-iolBCDEF-idh-iolHI-fbaB

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
mmsA yxdA - 4082920..4084383
COG1012C x1017-BAC  
iolB yxdB - 4082030..4082845
COG3718G  
iolC yxdC - 4081029..4082006
COG0524G iolC-BAC-1 iolC-BAC-2  
iolD yxdD - 4079083..4080996
COG3962E  
iolE yxdE - 4078173..4079066
COG1082G iolE-BAC  
iolF yxdF - 4076842..4078158
COG2814G  
idh iolG, iol, iol - 4074801..4075835 myo-inositol 2-dehydrogenase
idh-BAC idh-BAC  
iolH yxdG - 4074896..4075765
COG1082G  
iolI yxdH - 4073974..4074810
COG1082G  
fbaB iolJ, yxdI, yxdI - 4072097..4072969 fructose-1,6-bisphosphate aldolase
fbaA-BAC  

Operon evidence: S1 nuclease mapping of the 3' end; Northern blotting (11.5 kb transcript)
Reference: Yoshida K, et al. (1994), Yoshida KI, et al. (1997), Yoshida K, et al. (2000)
Comments: The proposed terminator agrees well with the experimentally determined termination site in the TTTTTTT stretch.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CcpA Negative +83:+110 4084465..4084492 TTTTTGAAAGCGTTTAATTCTTGGCTTG Miwa Y, et al. (2000): DP SDM
IolR Negative -107:-45 4084619..4084681 CCTTCTCTTACTTCTCTTACTTGATTAAAAGATTAATATAATAAAAATAATGAAAAAATGTAG Yoshida KI, et al. (1997): DB DP HB
IolR Negative -5:+17 4084558..4084579 TAACCAAGAAATGACCAAAAAG Yoshida KI, et al. (1999): GS FT
SigA Promoter -39:+8 4084567..4084613 ATGATTGACTTATGGGTATTATGCGATTAGAATATAACCAAGAAATG Yoshida KI, et al. (1997): PE
CcpA Negative ND 4082159..4082181 GAAATGAAAACGTTGTCATCGTT Miwa Y, et al. (2000): RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GAAACCGCCGAGATGGCGGTTTTTTTGTGCGGTG
     >>>>     <<<<
4073055..4073067
907..919
-8.1
 iolI

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai