Regulated Operon: | murE-mraY-murD-spoVE-murG-murB-divIB-ylxWX-sbp |
Genes | Synonyms | Direction | Genome position | Function | COG ID | Conserved groups |
---|---|---|---|---|---|---|
murE | + | 1586330..1587814 | COG0769M | murE-BAC murE-STA murE-STR | ||
mraY | + | 1587926..1588900 | COG0472M | |||
murD | + | 1588901..1590256 | COG0771M | murD-CLO murD-STR | ||
spoVE | + | 1590317..1591417 | COG0772D | spoVE-BAC | ||
murG | + | 1591540..1592631 | COG0707M | murG-BAC murG-STA | ||
murB | ylxC | + | 1592663..1593574 | COG0812M | murB-STR x1153-STA | |
divIB | tms-12 | + | 1593704..1594495 | COG1589M | ||
ylxW | + | 1594492..1595187 | COG3879S | |||
ylxX | + | 1595210..1595917 | COG3879S | |||
sbp | + | 1595935..1596300 | COG3856S | sbp-CLO |
Operon evidence: | Northern blotting (transcripts of 8.3, 6.0, 5.9, 4.9, 1.2, and 0.85 kb) |
---|---|
Reference: | Henriques AO, et al. (1992), Harry EJ, et al. (1994) |
Comments: | The stem-loop structure downstream of spoVE may function as a read-through terminator. Northern blotting results suggest that the stem-loop structure downstream of murB does not function as a transcriptional terminator. |
Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
---|---|---|---|---|---|
SigE | Promoter | -37:+6 | 1590218..1590260 | GGTGACATGTTTATAGATGCCGTGCATATGCTTAAGTAAGGGC |
Theeragool G, et al. (1993): PE S1 RG DB Miyao A, et al. (1993): RO, RNAP purification |
SigE | Promoter | -40:+9 | 1590077..1590125 | CTGGGAATACAACATGTCAAACGTGTCGATAATGTTGAACAAGCAGTAT |
Theeragool G, et al. (1993): PE S1 RG DB Miyao A, et al. (1993): RO, RNAP purification |
SpoIIID | Negative | -38:+21 | 1590079..1590137 | GGGAATACAACATGTCAAACGTGTCGATAATGTTGAACAAGCAGTATCTGCGGCGTTTG |
Eichenberger P, et al. (2004): FT AR |
SpoIIID | Negative | -38:+21 | 1590218..1590276 | GGTGACATGTTTATAGATGCCGTGCATATGCTTAAGTAAGGGCTTGTCTTGAAGTAAAT |
Eichenberger P, et al. (2004): FT AR |
SigA | Promoter | -50:+15 | 1593567..1593631 | ATCGCTGATTCAAGTTCTGACTGAAGCTGTTCATATGATATACTGTAAGCAAACGACAAACGGCA |
Harry EJ, et al. (1994): PE DB SDM RG DP NB |
Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
---|---|---|---|---|
GGAAACAATTGATGCGATAACCCTGTCCAACAAAACAGGGTTATCGTTATGTTATAGAGAA >>>>>>>>>>>> <<<<<<<<<<<< |
spoVE |
|