Regulated Operon: | pabBAC-sul-folBK-yazB-yacF-lysS |
Genes | Synonyms | Direction | Genome position | Function | COG ID | Conserved groups |
---|---|---|---|---|---|---|
pabB | pab | + | 82864..84276 | COG0147EH | pabB-BAC | |
pabA | trpX | + | 84290..84874 | COG0512EH | pabA-BAC x0931-STA | |
pabC | + | 84874..85755 | COG0115EH | |||
sul | + | 85737..86594 | COG0294H | |||
folB | yacE | + | 86587..86949 | COG1539H | ||
folK | + | 86946..87449 | COG0801H | |||
yazB | + | 87401..87610 | COG1476K | |||
yacF | + | 87631..88632 | x1602-BAC | |||
lysS | + | 88727..90226 | COG1190J | lysS-BAC-1 lysS-BAC-2 |
Operon evidence: | Northern blotting (7.5 kb transcript) |
---|---|
Reference: | De Sazieu A, et al. (1997), BSORF |
Comments: | Readthrough terminators after pabA (undefined) and yacF result in a 2.1 kb transcript and a 5.9 kb transcript, respectively. Promoter deletions, reporter gene fusions, and Northern blotting (1.5 kb transcript) suggested the existence of an internal promoter in front of lysS. The MtrB product (TRAP) causes translational attenuation at the pabA ribosomal binding site under conditions of excess tryptophan. |
Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
---|---|---|---|---|---|
SigA | Promoter | -43:+7 | 82788..82837 | GAAATTCACTTTTTTCACTAACAACATTGCTTTACAATTAAAAACAAGTA |
De Saizieu A, et al. (1997): PE RG NB |
MtrB | Negative | ND | 84236..84287 | GAGCATTAGAGCTGAGCGAAGAAGAGACAAAAATTAGATGAGGTGAGCGGAG |
Kane JF (1977): DB, anthranilate synthase activity, tryptophan excretion measurement Babitzke P, et al. (1994): filter binding assay Yang M, et al. (1995): RG DP SDM Lee AI, et al. (1996): DB RG Du H, et al. (1997): FT, in-vitro translation Valbuzzi A & Yanofsky C (2001): DB RG Yakhnin H, et al. (2004): RG DB GS |
Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
---|---|---|---|---|
AAAAAGAGCGGTATCCTCCATAGGGAAAGGATGCCGCTCTTTTTAAATCCCTTA >>>>>>>>>>>>>> <<<<<<<<<<<<<<<< |
lysS | |||
GCTCACTGCTAGTTTTACGCTGGCAGTTTTTCTGCTTTTTT >>>>>>>> <<<<<<<< |
yacF |
|