Regulated Operon: pabBAC-sul-folBK-yazB-yacF-lysS

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
pabB pab + 82864..84276
COG0147EH pabB-BAC  
pabA trpX + 84290..84874
COG0512EH pabA-BAC x0931-STA  
pabC
+ 84874..85755
COG0115EH  
sul
+ 85737..86594
COG0294H  
folB yacE + 86587..86949
COG1539H  
folK
+ 86946..87449
COG0801H  
yazB
+ 87401..87610
COG1476K  
yacF
+ 87631..88632

x1602-BAC  
lysS
+ 88727..90226
COG1190J lysS-BAC-1 lysS-BAC-2  

Operon evidence: Northern blotting (7.5 kb transcript)
Reference: De Sazieu A, et al. (1997), BSORF
Comments: Readthrough terminators after pabA (undefined) and yacF result in a 2.1 kb transcript and a 5.9 kb transcript, respectively. Promoter deletions, reporter gene fusions, and Northern blotting (1.5 kb transcript) suggested the existence of an internal promoter in front of lysS. The MtrB product (TRAP) causes translational attenuation at the pabA ribosomal binding site under conditions of excess tryptophan.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -43:+7 82788..82837 GAAATTCACTTTTTTCACTAACAACATTGCTTTACAATTAAAAACAAGTA De Saizieu A, et al. (1997): PE RG NB
MtrB Negative ND 84236..84287 GAGCATTAGAGCTGAGCGAAGAAGAGACAAAAATTAGATGAGGTGAGCGGAG Kane JF (1977): DB, anthranilate synthase activity, tryptophan excretion measurement
Babitzke P, et al. (1994): filter binding assay
Yang M, et al. (1995): RG DP SDM
Lee AI, et al. (1996): DB RG
Du H, et al. (1997): FT, in-vitro translation
Valbuzzi A & Yanofsky C (2001): DB RG
Yakhnin H, et al. (2004): RG DB GS

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAAGAGCGGTATCCTCCATAGGGAAAGGATGCCGCTCTTTTTAAATCCCTTA
     >>>>>>>>>>>>>>    <<<<<<<<<<<<<<<<
90234..90267
8..41
-25.3
 lysS
GCTCACTGCTAGTTTTACGCTGGCAGTTTTTCTGCTTTTTT
     >>>>>>>>     <<<<<<<<
88659..88679
27..47
-13.0
 yacF

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai