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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai |
| Regulated Operon: | purEKBCSQLFMNHD |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Conserved groups |
|---|---|---|---|---|---|---|
| purE | + | 698612..699100 | COG0041F | purE-BAC purE-STA purE-STR x1273-CLO | ||
| purK | + | 699093..700235 | COG0026F | |||
| purB | + | 700232..701527 | COG0015F | purB-STR | ||
| purC | + | 701601..702326 | COG0152F | purC-BAC purC-STR | ||
| purS | yexA | + | 702319..702573 | COG1828F | ||
| purQ | + | 702570..703253 | COG0047F | |||
| purL | + | 703237..705465 | COG0046F | x0046-STR | ||
| purF | purB | + | 705441..706871 | COG0034F | purB-STR | |
| purM | + | 706973..708013 | COG0150F | purM-BAC purM-STR | ||
| purN | + | 708010..708597 | COG0299F | |||
| purH | purJ | + | 708594..710132 | COG0138F | purH-BAC purH-STA purH-STR | |
| purD | + | 710148..711416 | COG0151F | purD-BAC purD-STA purD-STR |
| Operon evidence: | S1 nuclease mapping of 3' end (transcript ends at the T at position 710789); Northern blotting (12.8 kb transcript). About half of the transcripts terminate at the stemloop structure downstream of purF. S1 nuclease and reporter gene experiments showed that guanine can cause transcriptional termination 195 bp downstream of the transcription start site at the antiterminator structure in front of purE. Repression by adenine is mediated by PurR. |
|---|---|
| Reference: | Ebbole DJ & Zalkin H (1987), Ebbole DJ & Zalkin H: Genetics and biotechnology of Bacilli, vol.2 pp.51-55 (1988), Ebbole DJ & Zalkin H (1989), Yoshida K, et al. (2003) |
| Comments: |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| PurR | Negative | -91:-28 | 698279..698342 | AATTGATCTAAAACACGAACATTAGTAGAATGAATTTTTGTATCGTTCGATAATATCGTTGACA |
Ebbole DJ & Zalkin H (1987): S1 Ebbole DJ & Zalkin H (1989): RG Ebbole DJ & Zalkin H (1989): DP RG GS FT Saxild HH & Nygaard P (1991): Enzyme activity measurement Weng M, et al. (1995): GS FT DB DP RG Shin BS, et al. (1997): GS FT SDM Weng M & Zalkin H (2000): DB RG GS Saxild HH, et al. (2001): HM RG Bera AK, et al. (2003): GS DP, ultracentrifugation Xuan J, et al. (2005): GS SDM RG Qian J, et al. (2006): Classical genetics |
| SigA | Promoter | -45:+20 | 698325..698389 | TCGATAATATCGTTGACATTATCCATGTCCGTTGTTAAGATAAACATGAAATCAAAACACGACCT |
Ebbole DJ & Zalkin H (1987): S1 PE |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| CGATACATGTACCATGCCCGGTTTGTATTGCTTCCTCATAAGTGCAATGCAGAGCGGGTATTTTTTATTTTCTGA >>>>>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<<< |
purE | |||
| TGAAAAATGACATAAAGGCAGCGCAGTTCGGCTGCCTTTCTCTTTCTGCCC >>>>>> <<<<<< |
purF | |||
| GAAAACCCGCAGAATAGCTGCGGGTTTTTTGTTATCAAA >>>>>>> <<<<<<< |
purD |
|