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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai |
| Regulated Operon: | resABCDE |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Conserved groups |
|---|---|---|---|---|---|---|
| resA | ypxA | - | 2420804..2421343 | COG1225O | ||
| resB | ypxB | - | 2419179..2420807 | COG1333O | ||
| resC | ypxC | - | 2417984..2419159 | COG0755O | resC-BAC | |
| resD | ypxD | - | 2417181..2417903 | COG0745TK | resD-BAC-1 resD-BAC-2 | |
| resE | ypxE | - | 2415415..2417184 | COG5002T |
| Operon evidence: | Northern blotting |
|---|---|
| Reference: | Sun G, et al. (1996), Sorokin A, et al. (1993), Azevedo V, et al. (1993) |
| Comments: | Internal promoter in front of resD, leading to a 2.5 kb transcript. Azevedo found a 7.0 kb transcript that started upstream of ypuL (in front of resA), as well as a shorter 0.7 kb transcript corresponding to ypuL only. The transcripts detected by Azevedo and Sun both had a length of 7 kb, which is 1 kb longer than needed for a resABCDE operon. In addition, Azevedo found that a probe for ypuL hybridizes to the 7 kb transcript, indicating that the transcript corresponds to ypuL-resABCDE. Such a transcript agrees well with the length of 7 kb measured by Northern blotting. However, Sun's primer extension experiment showed a single promoter just in front of resA. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| PhoP | Positive | -72:-6 | 2421381..2421448 | ACGCTTTCTAAATTTCACATAACCTTCAAAAAGTAAGAAATGTGAAATGAACGTGCAATGATATAATT |
Birkey SM, et al. (1998): GS FT |
| ResD | Positive | ND | ND | ND |
Sun G, et al. (1996): DB Nakano MM, et al. (2001): RG |
| SigA | Promoter | -51:+4 | 2421371..2421425 | CTTCAAAAAGTAAGAAATGTGAAATGAACGTGCAATGATATAATTGAATGGATCT |
Sun G, et al. (1996): PE |
| PhoP | Negative | -45:+25 | 2417903..2417972 | CGACACAGTTCTCAAACTTTCTCACGATTTGATAAAATGAAAGTAACAGAAGGAAAGCAGGGGGAAAACA |
Birkey SM, et al. (1998): GS FT |
| SigA | Promoter | -43:+5 | 2417923..2417970 | ACACAGTTCTCAAACTTTCTCACGATTTGATAAAATGAAAGTAACAGA |
Sun G, et al. (1996): PE |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| AGAAACACCCGCTGACTGAGCGGGTGTTTTTTTAATAGCCA >>>>>>>> <<<<<<<< |
resE |
|