Regulated Operon: resABCDE

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
resA ypxA - 2420804..2421343
COG1225O  
resB ypxB - 2419179..2420807
COG1333O  
resC ypxC - 2417984..2419159
COG0755O resC-BAC  
resD ypxD - 2417181..2417903
COG0745TK resD-BAC-1 resD-BAC-2  
resE ypxE - 2415415..2417184
COG5002T  

Operon evidence: Northern blotting
Reference: Sun G, et al. (1996), Sorokin A, et al. (1993), Azevedo V, et al. (1993)
Comments: Internal promoter in front of resD, leading to a 2.5 kb transcript. Azevedo found a 7.0 kb transcript that started upstream of ypuL (in front of resA), as well as a shorter 0.7 kb transcript corresponding to ypuL only. The transcripts detected by Azevedo and Sun both had a length of 7 kb, which is 1 kb longer than needed for a resABCDE operon. In addition, Azevedo found that a probe for ypuL hybridizes to the 7 kb transcript, indicating that the transcript corresponds to ypuL-resABCDE. Such a transcript agrees well with the length of 7 kb measured by Northern blotting. However, Sun's primer extension experiment showed a single promoter just in front of resA.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
PhoP Positive -72:-6 2421381..2421448 ACGCTTTCTAAATTTCACATAACCTTCAAAAAGTAAGAAATGTGAAATGAACGTGCAATGATATAATT Birkey SM, et al. (1998): GS FT
ResD Positive ND ND ND Sun G, et al. (1996): DB
Nakano MM, et al. (2001): RG
SigA Promoter -51:+4 2421371..2421425 CTTCAAAAAGTAAGAAATGTGAAATGAACGTGCAATGATATAATTGAATGGATCT Sun G, et al. (1996): PE
PhoP Negative -45:+25 2417903..2417972 CGACACAGTTCTCAAACTTTCTCACGATTTGATAAAATGAAAGTAACAGAAGGAAAGCAGGGGGAAAACA Birkey SM, et al. (1998): GS FT
SigA Promoter -43:+5 2417923..2417970 ACACAGTTCTCAAACTTTCTCACGATTTGATAAAATGAAAGTAACAGA Sun G, et al. (1996): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AGAAACACCCGCTGACTGAGCGGGTGTTTTTTTAATAGCCA
     >>>>>>>>     <<<<<<<<
2415352..2415372
43..63
-16.9
resE

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai