Regulated Operon: rsbRSTUVW-sigB-rsbX

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
rsbR ycxR, ycxR + 518969..519793

 
rsbS ycxS + 520237..520602
COG1366T  
rsbT ycxT + 520606..521007
COG2172T  
rsbU
+ 521019..522026
COG2208TK rsbU-STA x0328-BAC  
rsbV
+ 522088..522417
COG1366T rsbV-BAC rsbV-STA  
rsbW
+ 522414..522896
COG2172T  
sigB rpoF + 522862..523650
COG1191K  
rsbX
+ 523650..524249
COG2208TK  

Operon evidence: S1 nuclease mapping of 3' end; lacZ fusion to rsbV; the promoter in front of rsbR was the only promoter detected in the rsbRSTU region
Reference: Kalman S, et al. (1990), Wise AA & Price CW (1995)
Comments: The S1 nuclease mapping showed that transcription ends in the T-stretch of the indicated terminator (positions 523844..523849). Internal promoter in front of rsbV.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -45:+21 519317..519382 AGAGCAACTTTTTTTGTTTTCAAAAAACATAAACGATATAATAGTGAAATAACGAAAAAATATGTT Wise AA, et al. (1995): PE
SigB Promoter -40:+3 522016..522058 GAAAGGTTTAACGTCTGTCAGACGAGGGTATAAAGCAACTAGT Kalman S, et al. (1990): PE S1 DB RG
Boylan SA, et al. (1993): PE; Western blot; RG
Voelker U, et al. (1994): 2D PAGE, HB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AAAAAGAAGCTGGACATCCGGCTTCTTTTTTTTGCGGTTG
     >>>>>>>>    <<<<<<<<
524263..524282
14..33
-13.5
rsbX

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai