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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai |
| Regulated Operon: | rsbRSTUVW-sigB-rsbX |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Conserved groups |
|---|---|---|---|---|---|---|
| rsbR | ycxR, ycxR | + | 518969..519793 | |||
| rsbS | ycxS | + | 520237..520602 | COG1366T | ||
| rsbT | ycxT | + | 520606..521007 | COG2172T | ||
| rsbU | + | 521019..522026 | COG2208TK | rsbU-STA x0328-BAC | ||
| rsbV | + | 522088..522417 | COG1366T | rsbV-BAC rsbV-STA | ||
| rsbW | + | 522414..522896 | COG2172T | |||
| sigB | rpoF | + | 522862..523650 | COG1191K | ||
| rsbX | + | 523650..524249 | COG2208TK |
| Operon evidence: | S1 nuclease mapping of 3' end; lacZ fusion to rsbV; the promoter in front of rsbR was the only promoter detected in the rsbRSTU region |
|---|---|
| Reference: | Kalman S, et al. (1990), Wise AA & Price CW (1995) |
| Comments: | The S1 nuclease mapping showed that transcription ends in the T-stretch of the indicated terminator (positions 523844..523849). Internal promoter in front of rsbV. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| SigA | Promoter | -45:+21 | 519317..519382 | AGAGCAACTTTTTTTGTTTTCAAAAAACATAAACGATATAATAGTGAAATAACGAAAAAATATGTT |
Wise AA, et al. (1995): PE |
| SigB | Promoter | -40:+3 | 522016..522058 | GAAAGGTTTAACGTCTGTCAGACGAGGGTATAAAGCAACTAGT |
Kalman S, et al. (1990): PE S1 DB RG Boylan SA, et al. (1993): PE; Western blot; RG Voelker U, et al. (1994): 2D PAGE, HB |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| AAAAAGAAGCTGGACATCCGGCTTCTTTTTTTTGCGGTTG >>>>>>>> <<<<<<<< |
rsbX |
|