Regulated Operon: | rsbRSTUVW-sigB-rsbX |
Genes | Synonyms | Direction | Genome position | Function | COG ID | Conserved groups |
---|---|---|---|---|---|---|
rsbR | ycxR, ycxR | + | 518969..519793 | |||
rsbS | ycxS | + | 520237..520602 | COG1366T | ||
rsbT | ycxT | + | 520606..521007 | COG2172T | ||
rsbU | + | 521019..522026 | COG2208TK | rsbU-STA x0328-BAC | ||
rsbV | + | 522088..522417 | COG1366T | rsbV-BAC rsbV-STA | ||
rsbW | + | 522414..522896 | COG2172T | |||
sigB | rpoF | + | 522862..523650 | COG1191K | ||
rsbX | + | 523650..524249 | COG2208TK |
Operon evidence: | S1 nuclease mapping of 3' end; lacZ fusion to rsbV; the promoter in front of rsbR was the only promoter detected in the rsbRSTU region |
---|---|
Reference: | Kalman S, et al. (1990), Wise AA & Price CW (1995) |
Comments: | The S1 nuclease mapping showed that transcription ends in the T-stretch of the indicated terminator (positions 523844..523849). Internal promoter in front of rsbV. |
Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
---|---|---|---|---|---|
SigA | Promoter | -45:+21 | 519317..519382 | AGAGCAACTTTTTTTGTTTTCAAAAAACATAAACGATATAATAGTGAAATAACGAAAAAATATGTT |
Wise AA, et al. (1995): PE |
SigB | Promoter | -40:+3 | 522016..522058 | GAAAGGTTTAACGTCTGTCAGACGAGGGTATAAAGCAACTAGT |
Kalman S, et al. (1990): PE S1 DB RG Boylan SA, et al. (1993): PE; Western blot; RG Voelker U, et al. (1994): 2D PAGE, HB |
Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
---|---|---|---|---|
AAAAAGAAGCTGGACATCCGGCTTCTTTTTTTTGCGGTTG >>>>>>>> <<<<<<<< |
rsbX |
|