Regulated Operon: sacXY

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
sacX sacS + 3942234..3943613
COG1263G  
sacY sacS + 3943667..3944509
COG3711K  

Operon evidence: lacZ gene fusion
Reference: Zukowski MM, et al. (1990), Glaser P, et al. (1990), Crutz AM & Steinmetz M (1992)
Comments: Binding of SacT (at low sucrose concentration) and by both SacT and SacY (at higher concentrations) to the ribonucleic antiterminator (RAT) sequence prevents formation of the terminator in the sacXY promoter, allowing transcription of the downstream sequence.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
DegU Positive -112:-56 3941835..3941891 GTCTTAAAGGTTTTTTTCATTCTAAGAACACCACACACAACCTTTTTCCCATCCATT Crutz AM & Steinmetz M (1992): DB RG DP
SacT Positive +19:+61 3941965..3942007 TATGGCGGGATTGTGACTGGGCAGGCAGGCAAGACCCAATGAT Tortosa P, et al. (1995): RG SDM DP
SacY Positive +19:+61 3941965..3942007 TATGGCGGGATTGTGACTGGGCAGGCAGGCAAGACCCAATGAT Tortosa P, et al. (1995): RG SDM DP
SigA Promoter -48:+20 3941899..3941966 CTTTTCATACTATTGCTATACAGCCATGAACAGCATAAAATGAACGTTATTACAGTTATCACCACATA Crutz AM & Steinmetz M (1992): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAACGCTTTTGATCATCTCAAAAGCGTTTTTTTATCTGATT
     >>>>>>>>>     <<<<<<<<<
3944523..3944545
14..36
-13.4
 sacY
ATTCTCATGCTCCGTCGTCGACGCGGGCATTTTTGTCATTTTCA
       >>>>>>>>>   <<<<<<<<<<
3942102..3942123
-1511..-1490
-13.1
 sacX

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai