Regulated Operon: spoVAABCDEF-lysA

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
spoVAA
- 2442684..2443304

 
spoVAB
- 2442269..2442694

 
spoVAC
- 2441804..2442256

spoVAC-BAC  
spoVAD
- 2440775..2441791

spoVAD-BAC  
spoVAE
- 2438993..2439964

 
spoVAF
- 2438372..2439853

 
lysA
- 2436947..2438266
COG0019E lysA-BAC  

Operon evidence: integrational plasmids; Northern blotting; downstream gene is in the opposite direction
Reference: Fort P & Errington J (1985), Azevedo V, et al. (1993), BSORF, Genbank L09228
Comments: The integrational plasmids showed that at least spoVAA through spoVAE belong to the same operon. The coding regions of spoVAE and spoVAF overlap. No terminator was found between spoVAF and lysA (Genbank L09228). Azevedo et al. found a 2.3 kb transcript orginating about 1 kb upstream of the lysA start codon, suggesting that transcription of spoVA continues into the lysA gene. However, the BSORF database contains a transcript consisting of the spoVA genes only. The lysA gene is also transcribed monocistronically as a 1.3 kb transcript.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigG Promoter -37:+21 2443311..2443368 GATGAATGAGAACAAAATCGAACCACATACTACATATATAACCACCGAAAGATGGTGA Moldover B, et al. (1991): PE
Sun DX, et al. (1989): DB RG
SpoVT Positive ND ND ND Bagyan I, et al. (1996): DB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AGAAAGCGCCGATTTTTCGGCGCTTTTCTTATTTGAAT
     >>>>>>>    <<<<<<<
2436923..2436940
7..24
-12.8
lysA

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai