Regulated Operon: spoVFAB-asd-dapG-dapA

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
spoVFA dpaA + 1744367..1745260
COG0686E spoVFA-BAC  
spoVFB dpaB + 1745263..1745865
COG0452H  
asd
+ 1745991..1747031
COG0136E asd-BAC asd-STA  
dapG lssD + 1747123..1748337
COG0527E  
dapA
+ 1748368..1749240
COG0329EM dapA-BAC x0966-BAC  

Operon evidence: primer extension analysis of dapG and dapA; integrative plasmids
Reference: Chen NY, et al. (1993), Daniel RA & Errington J (1993)
Comments: Internal promoter in front of asd. Second proposed terminator was found in a different section of the genome.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigK Promoter -40:+11 1744274..1744324 TTTTATATTCACCGGTATTTCCTTTTGATCATAAGATGAAGGGGAGCTTAA Chen NY, et al. (1993): PE
Daniel RA & Errington J (1993): RG PE DB
SigA Promoter -41:+18 1745895..1745953 AATTCGGTCATCACCCGCATATTCTATGGTAAAATAAAACGTAAAATCATAGTCAAATC Chen NY, et al. (1993): PE
unknown Promoter -46:+10 1745924..1745979 TAAAATAAAACGTAAAATCATAGTCAAATCAATTCAATGAAGCTGATTGGCGGAAG Chen NY, et al. (1993): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AAACGCGGTCATTCTCACATTCAGCTGAGTTTGACCGTTTCTTTTACATATT
     >>>>>>>>>>>>        <<<<<<<<<<<<
1749267..1749298
27..58
-15.6
dapA

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai