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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai |
| Regulated Operon: | srfAA-srfAB-comS-srfAC-srfAD |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Conserved groups |
|---|---|---|---|---|---|---|
| srfAA | comL | + | 376968..387731 | COG1020Q | ||
| srfAB | comL | + | 387744..398495 | COG1020Q | ||
| comS | + | 390880..391020 | ||||
| srfAC | comL | + | 398532..402359 | COG1020Q | ||
| srfAD | comL | + | 402388..403116 | COG3208Q |
| Operon evidence: | lacZ insertions |
|---|---|
| Reference: | Cosmina P, et al. (1993), Nakano MM, et al. (1991) |
| Comments: | The lacZ insertions show that the length of the operon is at least 25 kb, which covers srfAA-srfAB-comS-srfAC. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| CodY | Negative | -38:+49 | 376640..376726 | CGGTGATAAAAACATTTTTTTCATTTAAACTGAACGGTAGAAAGATAAAAAATATTGAAAACAATGAATAAATAGCCAAAATTGGTT |
Wray LV Jr, et al. (1996): DB FT GS |
| ComA | Positive | -118:-81 | 376560..376597 | TTGCGGCATCCCGCAAAAAATATTGCTGTAAATAAACT |
Hahn J & Dubnau D (1991): DB RG Nakano MM, et al. (1991): DP SDM Roggiani M & Dubnau D (1993): GS FT |
| ComA | Positive | -74:-37 | 376604..376641 | TTTCGGCATCCCGCATGAAACTTTTCACCCATTTTTCG |
Hahn J & Dubnau D (1991): DB RG Nakano MM, et al. (1991): DP SDM Roggiani M & Dubnau D (1993): GS FT |
| PerR | Positive | -163:-113 | 376515..376565 | TTTAAAAATTTTTATTTTTCTGTAAATAATGTTTAGTGGAAATGATTGCGG |
Hayashi K, et al. (2005): GS FT |
| SigA | Promoter | -46:+17 | 376632..376694 | CCATTTTTCGGTGATAAAAACATTTTTTTCATTTAAACTGAACGGTAGAAAGATAAAAAATAT |
Nakano MM, et al. (1991): PE |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| CAAAAGCGGACAGCTTCGGCTGTTCCGCTTTTTTTGTGTTGAA >>>>>>>>> <<<<<<<<<< |
srfAD |
|