Regulated Operon: valS-folC

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
valS
- 2866664..2869306
COG0525J valS-BAC valS-STA  
folC
- 2865312..2866604
COG0285H folC-STA  

Operon evidence: Northern blotting (4.4 kb transcript)
Reference: Grundy FJ & Henkin TM (1993), Luo D, et al. (1997), Genbank L04520, Genbank X77239
Comments: The readthrough terminator downstream of valS leads to a 3.0 kb monocistronic valS transcript. Based on homology, transcriptional readthrough is expected to occur at the stem-loop upstream of valS if uncharged valine tRNA binds to the T-box motif in the nascent mRNA transcript. The tRNA anticodon binds to the GUC codon in the sequence AAGGUCGCC in the valS leader mRNA.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -43:+8 2869611..2869661 AGATGATTGACGAATCAAAAACACTTTTACTATAATAAAGAACATAAATAA Luo D, et al. (1997): PE NB
TrnSL-Val2 Positive ND 2869375..2869417 GATTGAGTTCATGAAAAAAGGTGGTACCGCGAAAGAGCTTTTC Grundy FJ & Henkin TM (1993): HM
Luo D, et al. (1997): RG SDM OV

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
TACCGCGAAAGAGCTTTTCGTCCTTTTACAGGGATGAAGAGCTCTTTTTTCGTTCTCAG
          >>>>>>>>>>>>>>      <<<<<<<<<<<<<<
2869350..2869383
-2719..-2686
-23.5
 valS
GGCTCCGGCAGAATCAAAAAAAGATTCTGCCGTTTTTTTCATGTGTA
     >>>>>>>>>>       <<<<<<<<<<
2865272..2865298
14..40
-18.1
 folC
GCCGGGGCGGTTTCTAGAGAAACCGCCTTGTCTTATAAAAGA
     >>>>>>>>>    <<<<<<<<<
2866631..2866652
12..33
-17.1
 valS

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai