Regulated Operon: ytmIJKLMNO-ytnIJ-ribR-ytnLM

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
ytmI
- 3008249..3008785
COG0456R  
ytmJ tcyJ - 3006465..3007274

 
ytmK tcyK - 3005639..3006451

 
ytmL tcyL - 3004898..3005617

 
ytmM tcyM - 3004161..3004868

 
ytmN hisP, tcyN, hisP - 3003385..3004164

 
ytmO
- 3003345..3004349
COG2141C  
ytnI
- 3003049..3003330
COG0695O  
ytnJ
- 3001724..3003052
COG2141C  
ribR ytnK, ytnK - 3000024..3000716 riboflavin kinase
 
ytnL hipO - 2999717..3000967
COG1473R  
ytnM
- 2998796..2999698
COG0730R  

Operon evidence: Northern blotting (transcript length between 10 kb and 11 kb)
Reference: Solovieva IM, et al. (2004), Solovieva IM, et al. (2005)
Comments:

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
YjbD Negative ND ND ND Erwin KN, et al. (2005): PE RG DB
YtlI Positive -76:-31 3008872..3008917 ATCATTAAAATTACTGAAGTGAAGGTTTACTTTTTTTGATTAGTCA Coppee JY, et al. (2001): DB
Burguiere P, et al. (2005): RG DP DB SDM GS
YrzC Negative -5:+37 3008805..3008846 AAACAATAAAAGCTTAGTAAACAAATAGGAATTATATATTTT Solovieva IM, et al. (2005): promoter mutations, riboflavin synthesis, DB OV, dot-blot analysis
Burguiere P, et al. (2005): RG DB
SigA Promoter -54:+21 3008822..3008896 AAGGTTTACTTTTTTTGATTAGTCAAAGATCAGAGTATGCTTTATAGTATAAACAATAAAAGCTTAGTAAACAAA Burguiere P, et al. (2005): PE
Erwin KN, et al. (2005): PE
Solovieva IM, et al. (2004): NB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
TGTAAGAAGCCGGTCGGGCTTCTTTTTTATTTTCCAG
     >>>>>>     <<<<<<
2998705..2998721
75..91
-10.6
 ytnM

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai