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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | cggR-gapA-pgk-tpiA-pgm-eno |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| cggR | yvbQ | - | 3481783..3482805 | transcriptional regulator | COG2390 | |
| gapA | gap | - | 3480729..3481736 | glyceraldehyde-3-phosphate dehydrogenase | COG0057 | gapA-BAC-1 gapA-MYC-1 gapA-BAC-1 gapA-MYC-1 gapA-MYC-2 stu1788-STA stu1788-STR |
| pgk | gerG | - | 3479228..3480412 | phosphoglycerate kinase | COG0126 | pgk-BAC pgk-CLO pgk-LAB pgk-STA |
| tpiA | tpi, tim | - | 3478436..3479197 | triose phosphate isomerase | COG0149 | tpi-BAC tpi-LAB tpi-STA tpi-STR tpi-BAC tpi-STA tpi-STR |
| pgm | - | 3476908..3478443 | phosphoglycerate mutase | COG0696 | x0574-STR | |
| eno | - | 3475586..3476878 | enolase | COG0148 | eno-BAC eno-MYC eno-STA eno-STR |
| Operon evidence: | Northern blotting (7.4 kb transcript) |
|---|---|
| Reference: | Ludwig H, et al. (2001), Genbank L29475 |
| Comments: | The readthrough terminator downstream of gapA leads to a 2.2 kb cggR-gapA transcript and a 6.2 kb gapA-pgk-tpiA-pgm-eno transcript. The internal promoters in front of gapA and pgk lead to a 1.2 kb monocistronic gapA transcript and a 4.8 kb pgk-tpiA-pgm-eno transcript. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| CggR | Negative | +27:+72 | 3482830..3482875 | CTATGACGGGACGTTTTTTGTCATAGCGGGACATATAATGTCCAGC |
Kodama T, et al. (2000): PE RG Doan T, et al. (2003): DB FT |
| SigA | Promoter | -42:+14 | 3482888..3482943 | CTTAACAGTTGAATAAACAATTCCACCCTGTTAAAATAATTAAAGAAAGCAGAAAT |
Ludwig H, et al. (2001): PE |
| SigA | Promoter | -49:+9 | 3480659..3480716 | AAAGGACCTGACTTGGTTCTTTCGAATAGAAGCGCTATAATGAAAGCGGACAAGGGAA |
Ludwig H, et al. (2001): PE |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| AAAGACCGCCGGGATTTTCTCTCGGCGGCTTTTTTATGCTTTCA >>>>>>>>> <<<<<<<<< |
eno | |||
| GACAAGGGAAGGGGACGGACTCCCTTTCCCTTTTTCCATGAAGAC >>>>>>>>>> <<<<<<<<<< |
gapA |
|