Regulated Operon: cotYZ

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
cotY
- 1249328..1249816 spore coat protein (insoluble fraction)
 
cotZ
- 1248754..1249200 spore coat protein (insoluble fraction)
cotZ-BAC  

Operon evidence: Northern blotting (1.4 kb transcript)
Reference: Zhang J, et al. (1994), Zhang J, et al. (1993)
Comments: The readthrough terminator or mRNA processing site downstream of cotY leads to a 0.6 kb transcript.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
GerE Positive -57:-34 1249889..1249912 TGAATATATAGACGTTCACCCACA Zhang J, et al. (1994): FT RO
GerE Positive -49:-33 1249888..1249904 TAGACGTTCACCCACAC Zhang J, et al. (1994): FT RO
SigK Promoter -44:+4 1249852..1249899 GTTCACCCACACCAAGTGGGGCACGGGTACATATGTTGTTAAGGACTA Zhang J, et al. (1994): NB PE DB RO
YlbO Positive ND ND ND Kuwana R, et al. (2005): SDS NB DB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GAAAAGCTTGCAGGCCTGCAGGCTTTTCTCTATGTAAA
     >>>>>>>    <<<<<<<
1248721..1248738
16..33
-11.6
 cotZ
GTAAAAAAAGCTGTCCGGCAACATCAGCCGGGCAGCTTTTTTCTTTTTCGT
         >>>>>>>>>>       <<<<<<<<<<
1248675..1248701
53..79
-21.6
 cotZ
ATCATTCAGCTGCTTCCAGCGTGAGGCAGCTGACTCTCCATCATTTCGC
     >>>>>>>>>>>      <<<<<<<<<<<
1249249..1249276
52..79
-21.4
 cotY

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai